Acquisition and Operation of a Light Microscopy and Digital Imaging Facility

卡尔9724473这项提案描述了芝加哥大学的共用共焦显微镜设施，它将为广泛的科学家开辟新的研究路线。为了满足用户群的即时和预期需求，拟议的设施将包括两个独立的系统，一个蔡司LSM共焦系统具有互补的优势，但两者都能够产生高分辨率的共焦图像，适合固定或活标本。数据集的可视化将使用Macintosh PowerPC和Silicon Graphics计算机以及用于图像分析和显示的相关软件来完成。由于生物成像和可视化在很大程度上仍然是一项“亲力亲为”的事业，而且在很大程度上仍然是技术性的，拟议中的设施旨在提供一个“用户友好”的环境，使个人研究人员、他们的受训人员和学生能够接触到这项技术。为此，该设施将配备一名全职员工，其任务是培训和协助希望利用这些强大机器能力的研究人员。

Karr 9724473 This proposal describes a shared confocal microscopy facility at the University of Chicago that will open new lines of research for a broad range of scientists. To meet the immediate and anticipated demands of the user base, the proposed facility will include two stand-alone systems, a Zeiss LSM confocal systems have complementary strengths, but both are capable of producing high-resolution confocal images and are suitable for either fixed or living specimens. Visualization of datasets will be accomplished using Macintosh PowerPC and Silicon Graphics computers and associated software for image analysis and display. Because biological imaging and visualization is still very much a "hands on" enterprise and remains for the most part quite technical, the proposed facility is designed to provide a "user friendly" environment to made the technology accessible to individual investigators, their trainees and students. To this end, the facility will be a staffed by an FTE whose mission will be to train and assist researchers who wish to utilize the capabilities of these powerful machines.