U.S.-Argentina Cooperative Research: Rab GTPases and Membrane Fusion

9724821斯塔尔 该美洲计划奖将资助密苏里州圣路易斯市华盛顿大学的Philip斯塔尔博士和阿根廷门多萨组织学和胚胎学研究所（IHEM）的Marisa科隆博博士之间的合作研究项目。他们将使用体外和体内方法进行实验，以确定关键蛋白质在控制吞噬作用中的可能参与，吞噬作用是细胞内化大颗粒的过程。 这些颗粒，通常包括活的微生物，被隔离在称为吞噬体的膜结合区室中。 该提案的重点是了解吞噬体-内体融合和吞噬体成熟的机制。 体外重建试验将用于剖析调节膜融合和转运的分子机制。 这项技术是在华盛顿大学的PI实验室开发的，目前正在由门多萨的阿根廷合作者进一步开发和完善 这是一个重要的基础研究领域，可以阐明吞噬过程中的基本分子机制，为未来抗感染策略的发展所必需。 ***

9724821 Stahl This Americas Program award will fund a collaborative research project between Dr. Philip Stahl, Washington University, St. Louis, Missouri, and Dr. Marisa Colombo, Institute for Histology and Embryology (IHEM), Mendoza, Argentina. They will conduct experiments, using in vitro and in vivo approaches, to determine the possible involvement of key proteins in controlling phagocytosis, the process by which cells internalize large particles. These particles, often including live microorganisms, are sequestered within membrane-bound compartments called phagosomes. The focus of the proposal is to develop an understanding of the mechanism of phagosome-endosome fusion and phagosome maturation.. In vitro reconstitution assays will be used to dissect the molecular mechanisms that regulate membrane fusion and transport. This technology, developed in the PI laboratory in Washington University, is being further developed and refined by the Argentinean collaborator in Mendoza This is an important area of basic research that could elucidate the fundamental molecular mechanisms during phagocytosis necessary for the future development of anti- infective strategies. ***