Optimized Ultraviolet Fluorescence Detection of Biological Chromophores

化学系的这一启动补助金授予了 德克萨斯大学奥斯汀分校支持杰森B教授的研究。切变 的 本研究的主题是紫外荧光检测生物 发色团 这项工作调查的机制和程度， 多光子激发的单胺类神经递质降解 使用不同数量的不同特性的光子 波长 比较了它们的光破坏量子产率 使用锁模的三光子激发神经递质 钛：蓝宝石激光器在近红外波长范围内， 使用倍频钛：蓝宝石激光器的双光子激发。 双光子和三光子激发荧光的背景来源 评估测量结果并与 优化紫外荧光的光破坏测量 天然荧光神经递质的检测， 毛细管电泳 这项研究将推动定量测量的努力， 亚细胞水平的神经化学。 提高了对 各种天然荧光神经递质将提供新的 表征化学基本机理的能力 细胞之间的信号。

This starter grant award of the Chemistry Division to the University of Texas at Austin supports the research of Professor Jason B. Shear. The theme of the research is ultraviolet fluorescence detection of biological chromophores. The work investigates the mechanisms and extent of multiphoton-excited degradation of monoamine neurotransmitters excited using different numbers of photons of different characteristic wavelengths. Comparisons are made of the photodestruction quantum yields for three-photon excitation of neurotransmitters using a modelocked titanium:sapphire laser in the near-infrared wavelength range, and for two-photon excitation using a frequency doubled titanium:sapphire laser. Sources of background in both two- and three-photon excited fluorescence measurements are evaluated and used in combination with the photodestruction measurements to optimize ultraviolet fluorescence detection of natively fluorescent neurotransmitters analyzed with capillary electrophoresis. The research will advance efforts toward quantitative measurement of neuronal chemistry at the subcellular level. Improved detectability of a variety of natively fluorescent neurotransmitters will provide new capabilities for characterizing fundamental mechanisms of chemical signalling between cells.