CAREER: High-speed Electrophoretic Imaging of Biological Microenvironments

这个由分析和表面化学项目支持的教师早期职业发展（Career）项目，侧重于开发具有高灵敏度检测策略的高速分离技术，以研究单细胞的神经递质释放。德克萨斯大学奥斯汀分校的Shear教授和他的学生将制造一种新型的多通道毛细管电泳阵列，用于快速采样和分离排泄的神经递质。多光子激发荧光可以从极小（亚皮升）的体积中产生，在保持色谱完整性的同时提供高灵敏度。这种组合将允许通过单个细胞的“景观”监测神经递质释放。这个职业研究项目将使本科生接触到现代研究实验室仪器，并通过分析“现实世界”的样本来说明这些方法的实用性。Shear教授将在他的讲座中结合化学仪器的基本方面和在实验室中动手解决问题。神经化学领域长期存在的问题之一是希望在单细胞水平上实时观察神经递质释放。德克萨斯大学奥斯汀分校的Shear教授和他的学生正在开发一种基于新型毛细管电泳阵列和高灵敏度光学光谱方法相结合的监测技术。具有监测细胞表面单个神经递质释放能力的仪器将影响许多学科。该项目仪器开发部分的多学科方面与Shear教授的兴趣相辅相成，他希望向本科生介绍最先进的研究仪器，以解决可能具有高度复杂性的“现实世界”分析问题。│Á萨拉伊特À萨拉伊特/ /¥Á¢│职权范围Á│Á│%/¢Á¢½Á_职权范围│？╝/ ?鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美鲜美的鲜美。￥/¥│%/¢¢？ Á→_/¥Á→╣/%¢¼©Á’？ÂÂÁ萨拉萨拉对Á ？╝¥┐╣¥╣一¢¥吗?■¥- '  - /_Á¥/% 00% 00% ？烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧烧？一个│©现代╣¢¥╝”║©”¢╣│¢/┤_ /¥╝╣/ %¢¢│╣│一/¢┴ %% /¢ _/` ║吗?¥一¥╣/ % /║║%╣│/¥╣吗?¢-│©/¢？%/某某ÁÁ某某À’││Á某某¢╣？┴/¢¥┤AA╝/┤/¥╣吗?/┤? %│¥╝吗?╣│¢¼©Á_¥©Á¢Á¢¥- Á¢%©/流星Á / ？￥/￥╣_ _ _ _ /│￥？¥©/║║%╣│/¥╣吗?？一个¢现代╣│?┤┐│¥吗?╝/ ?_ / 0.00￥职权范围│%Á¢职权范围│Á¥©Á职权范围│一¢¢一¢¥吗?▓一¢¥┐┤╣一┤┤一个¥╝_╣¥©一个AAA╣│╣一│' /┤¥©一│©现代╣│/ %¢¥/▓╣%╣¥”? /′′│Á│╣Á¢_/↓_¢-│©/？_ / 0.00￥╣│Á¾Á¾%¥¾Â¾？_¥©╣¢¢¥-│’→╣%% /%¢？/┤┤¥吗?¥©Á ÀÁÁ <e:1> /% -│Á <e:1>¢¥/ À ？Â¥©Á ' /_╣│¢╣¥©Á¢？%╣┤%╣╗┐╣┤╣¥一个╝/│┴╣│©╣¢一¢¢一¥╣/ % ?<s:1>¥©Á <e:1> Á¢╣À /？ _/ '咋_ _ _ _ _ _ ？<s:1>¥/¥Á│╣Á¢/ ？│Á¢¢Á¢-│©/¢¥©Á¢？%/ 0.00│Á%% Á%Á│¥0.00 ？│©Á_╣¢¥某某' /©Á¥Á某某？ÀÁÁ？┐¢│¥/ %’¢╣¢

This Faculty Early Career Development (CAREER) project, supported in the Analytical and Surface Chemistry Program, focuses on the development of high speed separation techniques with high sensitivity detection strategies to study neurotransmitter release from single cells. Professor Shear and his students at the University of Texas-Austin will fabricate a novel, multichannel capillary electrophoresis array for the rapid sampling and separation of excreted neurotransmitters. Multiphoton-excited fluorescence can be generated from extremely small (sub-picoliter) volumes to provide high sensitivity while retaining chromatographic integrity. This combination will allow the monitoring of neurotransmitter release across the 'landscape' of a single cell. This CAREER research project will expose undergraduate students to modern research laboratory instrumentation and illustrate the utility of the approaches through the analysis of `real world` samples. Professor Shear will combine fundamental aspects of chemical instrumentation in his lectures with hands on problem solving in the laboratory. One of the long standing issues in the area of neurochemistry has been the desire to observe neurotransmitter release on the single-cell level in real time. Professor Shear and his students at the University of Texas-Austin, are developing monitoring techniques based on the combination of a novel capillary electrophoresis array and high sensitivity optical spectroscopy methods. Instrumentation having the capabilities to monitor individual neurotransmitter releases across the surface of a cell would impact a number of disciplines. The multidisciplinary aspects of the instrumentation development portion of this project are complemented by Professor Shear's interest in introducing undergraduates to state-of-the-art research instrumentation for solving `real world` analytical problems which may have a high degree of complexity. ┤Á╝À╝/┤┐/¥Á ¢│╣Á│Á │%/¢¢Á¢ ½Á_╣│?┤┐│¥?╝ /?║/╝¥╣│%Á¢ /╝Á / ╣_║?╝¥/¥ │%/¢¢ ? Á ┴ _/¥Á╝╣/%¢ ¼©Á` ?ÂÂÁ╝ ┐╣╗┐Á ?║║?╝¥┐╣¥╣Á¢ ¥? ¢¥┐┤` Â┐┤/_Á¥/% ║╝?▓%Á_¢ ╣ ¥©Á ╣¥Á╝┤╣¢│╣║%╣/╝` /╝Á/¢ ? │©Á_╣¢¥╝` ║©`¢╣│¢ /┤ _/¥Á╝╣/%¢ ¢│╣Á│Á /¢ ┴Á%% /¢ _/` ║?¥Á¥╣/% /║║%╣│/¥╣?¢ ¢┐│© /¢ ¢?%/╝ ÁÁ╝À` │?└Á╝¢╣? ┴/¢¥Á ┤ÁÀ╝/┤/¥╣? /┤ /?Á%Á│¥╝?╣│¢ ¼©Á ┤Á¥/╣%Á┤ ┐┤Á╝¢¥/┤╣À ╝Á¢┐%¥╣À Â╝?_ ¥©Á¢Á ¢¥┐┤╣Á¢ ┴╣%% ©/└Á / ╣_║?╝¥/¥ ╣_║/│¥ ? ¥©Á /║║%╣│/¥╣? ? ¢Á_╣│?┤┐│¥?╝ /?║/╝¥╣│%Á¢ ╣│Á ¥©Á ║╝?│Á¢¢Á¢ ¥? ▓Á ¢¥┐┤╣Á┤ ┤Á¥Á╝_╣Á ¥©Á ÁÂÂ╣│╣Á│` /┤ ¥©Á │©Á_╣│/% ¢¥/▓╣%╣¥` ? /` ┤Á└╣│Á¢ _/┤Á Â╝?_ ¢┐│© /?║/╝¥╣│%Á¢ ¬Á¢┐%¥¢ Â╝?_ ¥©╣¢ ¢¥┐┤` ┴╣%% /%¢? /┤┤ ¥? ¥©Á ÀÁÁ╝/% ┐┤Á╝¢¥/┤╣À ? ¥©Á ┤`/_╣│¢ ╣ ¥©Á ¢?%╣┤ %╣╗┐╣┤ ╣¥Á╝Â/│Á ┴╣│© ╣¢ Á¢¢Á¥╣/% Â?╝ ¥©Á ┤Á¢╣À /┤ /║║%╣│/¥╣? ? _/` ╣_║?╝¥/¥ ┤Á└╣│Á¢ /┤ ║╝?│Á¢¢Á¢ ¢┐│© /¢ ¥©Á ¢?%/╝ │Á%% Á%Á│¥╝?│©Á_╣¢¥╝` /┤ ©Á¥Á╝?ÀÁÁ?┐¢ │/¥/%`¢╣¢