SBIR Phase I: Fiber Optic Affinity Ligand Sensor for Quantification of Petroleum and Bioremediation

*9760865琼斯这个小型企业创新研究第一阶段项目将解决石油勘探、生产、提炼和储存地点周围环境的污染问题。生物修复技术已被用于从环境中去除有害化学物质；然而，为了获得必要的敏感性和特异性，需要昂贵且耗时的技术来阻碍对这些地点的监测。例如，已使用气相色谱与质谱联用(GCMS)、毛细管区带电泳(CZE)或高效液相色谱(HPLC)来监测环境暴露，并使用各种微生物活性和生物量分析方法来监测修复过程。GCIMS、CZE和高效液相色谱仪需要昂贵的仪器、广泛的操作员专业知识和从污染现场移走的实验室分析。微生物活性或生物量分析是耗时的，对感兴趣的微生物的特异性也不可靠。第一阶段将探索一种新型光纤传感器，该传感器具有在石油活动领域及其化学和生物传感平台上进行环境监测的潜力。生物和化学传感器有望产生高分辨率、低成本的多目标系统，用于1)环境监测、2)饮用水和废水监测、3)医疗领域的大规模、高速测试、4)化学分析和5)先进材料加工的智能监测。***

*** 9760865 Jones This Small Business Innovation Research (SBIR) Phase I project will address contamination in environments surrounding sites of petroleum exploration, production, refinement, and storage. Bioremediation techniques have been used to remove hazardous chemicals from the environment; however, monitoring these sites is hindered by expensive and time consuming techniques required to obtain necessary sensitivity and specificity. For example, gas chromatography coupled with mass spectrometry (GCMS), capillary zone electrophoresis (CZE), or high performance liquid chromatography (HPLC) have been used to monitor environmental exposure, and various methods of microbial activity and biomass assays have been used to monitor remediation processes. GCIMS, CZE, and HPLC require expensive instrumentation, extensive operator expertise, and laboratory analysis removed from the site of contamination. Microbial activity or biomass assays are time consuming, and specificity for the microbe of interest is not reliable. Phase I will explore a novel optical fiber sensor that has the potential for environmental monitoring in areas of petroleum activity and its in a chemical and biological sensing platform. Biological and chemical sensors are expected to yield high-resolution, low-cost, multi-target systems for applications in 1) environmental monitoring, 2) drinking and wastewater monitoring, 3) large scale, high-speed testing in the medical field, 4) chemical analysis, and 5) intelligent monitoring of advanced materials processing. ***