Development of a Cell Culture Facility for Undergraduate Investigations in Eukaryotic Gene Expression

该项目的主要重点是对现有的细胞生物学、病毒学和重组基因技术实验室进行升级。其他课程，如动物生理学、免疫学、植物生理学和独立的本科生研究项目也受益于该设施。有了新的细胞培养设施，学生们能够使用动物和植物细胞来研究细胞事件和基因表达。病毒学在其教学大纲中增加了一门实验室课程。重组基因技术(RGT)是一项专门的计划；是该州唯一的一项此类计划，也是全国少数几项计划之一。细胞培养设备支持RGT过程中真核基因表达的实验室研究。结果正在通过州和国家会议传播。正在分发实验室手册、网站和出版物。目前针对生物专业本科生的评估测试和学生评价/反馈调查正在进行修改，以分析该项目的成功。这一设施的增加为学生提供了更广泛的经验，特别是为肯塔基州的学生做好了准备，他们通常是第一代大学毕业生，拥有在该州和全国范围内竞争职位所需的实验室技能。

The major focus of this project is an upgrade of existing laboratories for cell biology, virology and recombinant gene technology. Other courses such as animal physiology, immunology, plant physiology, and independent undergraduate research projects also benefit from this facility. With the new cell culture facility, students are able to use animal and plant cells to study cellular events and gene expression. Virology has added a laboratory component to its syllabus. Recombinant gene technology (RGT) is a specialized program; the only one of its kind in the state and one of a handful in the nation. A cell culture facility supports laboratory study of eukaryotic gene expression in the RGT course. Results are being disseminated through state and national meetings. Laboratory manuals, web-site and publications are being distributed. Current assessment tests for undergraduate biology majors and student evaluation/feedback surveys are being modified to analyze success of this project. The addition of this facility provides a greater breadth of experience for students, and especially prepares Kentucky students, who are often first generation college graduates, with the laboratory skills needed to compete for positions both within the state and across the nation.