SBIR Phase I: In Situ Infrared Analyzer for Multiple Components in Bioreactors

这个小企业创新研究（SBIR）第一阶段项目将开发一个多组件中红外分析仪系统，其中包括一个创新的原位内部反射探头，该探头与红外光谱仪具有柔性耦合。探针将通过衰减全反射（ATR）晶体元件中的倏逝场相互作用对生物反应器发酵液中的多种组分进行采样。柔性耦合元件的红外光通达率高于传统的卤化物红外纤维，并且元件在机械上更坚固。该分析仪还包含一个自清洁过滤机构，以防止活性探针表面的生物污染。在I期工作中，该分析仪将监测生物反应器系统中的甘油和醋酸盐浓度，总测量周期为1分钟，灵敏度可达1 g/l。红外分析仪系统将在生物技术，制药和食品工业中使用的生物反应器系统中找到商业应用。该分析仪将为这些行业提供改进的过程控制和效率。提出的原位红外多组分分析仪将为生物技术、制药和食品工业的生物反应器提供有价值的过程控制和诊断工具。它还将在石油和化学原料工业中得到应用。

9860867 This Small Business Innovation Research (SBIR) Phase I project will develop a multi-component mid-infrared analyzer system which includes an innovative in-situ internal reflection probe with a flexible coupling to an infrared spectrometer. The probe will sample multiple components in the fermentation broth of a bioreactor via evanescent field interaction in an attenuated total reflection (ATR) crystal element. The infrared light throughout of flexible coupling elements is higher than conventional, halcogenide infrared fibers and the elements are mechanically more robust. The analyzer also contains a self-cleaning filter mechanism to prevent biofouling of the active probe surface. In the Phase I effort, the analyzer will monitor glycerol and acetate concentrations in a bioreactor system with a one minute total measurement cycle time and sensitivity down to 1 g/l range. The Infrared analyzer system will find commercial applications in bioreactor systems used in the biotechnology, pharmaceutical, and food Industries. The analyzer will provide improved process control and efficiency in these industries. The proposed in situ infrared multiple component analyzer will provide a valuable process control and diagnostics tool for use in bioreactors in the biotechnology, pharmaceutical, and food industries. It will also find applications in the petroleum and chemical feedstock Industries.