CAREER: Effect of Pretreatment on Inactivation of Cryptosporidium in Natural Waters

9875580哈林顿：这个项目的目的是调查水处理过程中降低消毒剂浓度的影响，消毒剂需要天然有机物等物质，对消毒剂(如臭氧和氯)灭活水中具有致病意义的隐孢子虫物种的效果有影响。研究人员计划使用完全混合的流通式反应器来评估隐孢子虫卵囊的灭活，作为开发灭活过程的数学模型的一步，这些数学模型可用于水处理过程的工程设计。这项研究将与威斯康星州立卫生实验室合作进行，该实验室将提供所用的隐孢子虫种类，并进行生存能力测定。将使用的联合活性染料/流式细胞术方法将允许进行比其他替代方法(如观察动物感染性)更大的实验矩阵。获得这一奖项的建议是为了响应教师早期职业发展(CALEAR)计划，该计划强调将新知识的发现与鼓舞人心的教学和热情学习相结合。该项目的研究部分预计将提供可应用于从供水中灭活隐孢子虫物种的程序和系统的工程设计方面的知识。预计教育部分将为本科生和研究生提供研究水处理微生物灭活过程的技术，这些技术在环境工程实践中具有重要意义。该调查员在咨询实践中的私营部门经验预计将为学生提供将研究结果与工程实践中的实用联系起来、培养创造性思维和强调沟通技能的实践经验。***

9875580 Harrington The objective of this project is to investigate the influence of water treatment processes that reduce concentrations of disinfectant demanding substances such as natural organic matter on the effectiveness of disinfectants such as ozone and chlorine for inactivation of cryptosporidium species of pathogenic significance in water. The investigator plans on using completely mixed flow-through reactors to evaluate inactivation of cryptosporidium oocysts as a step toward development of mathematical models of the inactivation processes that can be used in engineering design of water treatment processes. The research will be conducted in cooperation with the Wisconsin State Laboratory of Hygiene, which will provide the cryptosporidium species used and will perform determinations of viability. The combined vital dye/flow cytometry approach to be used will permit a larger matrix of experiments than would be possible with other alternatives such as observartions of animal infectivity. The proposal leading to this award was submitted in response to the Faculty Early Career Development (CAREER) Program which emphasizes combining discovery of new knowledge with inspired teaching and enthusiastic learning. the research component of this project is expected to provide knowledge that can be applied in engineering design of processes and systems to inactivate cryptosporidium species from water supplies. The educational component is expected to provide undergraduate and graduate students with access to techniques for study of microbial inactivation processes for water treatment of significance in environmental engineering practice. The investigator's private-sector experience in consulting practice is expected to provide students with practical experiences in relating results of research to utility in engineering practice, in fostering creative thinking and emphasizing communication skills. ***