CAREER: Analytical Applications of MALDI Mass Spectrometry to the Study of Surface-Protein Interactions

该职业奖授予德克萨斯大学阿灵顿分校的加里金塞尔教授，由化学系的分析和表面化学项目支持。 拟议的工作的主要研究目标是开发一种新的系统方法，用于研究生物分子表面相互作用，形成基质辅助激光解吸电离实验的核心。 表面准备在一些新的方法，包括光束的方法，然后测量的MALDI响应，以开发一个解释性的框架。 除了直接应用到改进的MALDI分析，应用程序来了解接触吸附和生物污损等表面性质的设想。 该提案的教育方面侧重于在该机构材料科学项目的主持下开发实验室课程和指导计划。基于激光的质谱分析方法取决于适当的样品制备。 加里·金塞尔所描述的工作将建立金属表面和蛋白质生物分子之间的相互作用类型，这些分子通常通过基于激光的质谱法进行分析，并提供样品制备所需的信息，从而提高分析的灵敏度和特异性。

This CAREER award to Professor Gary Kinsel of the University of Texas at Arlington is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The primary research objective of the proposed work is the development of a new systematic approach for the study of biomolecular surface interaction that forms the core of the matrix-assisted laser desorption ionization experiment. Surfaces are prepared in a number of novel methods, including beam approaches, and then the MALDI responses measured in order to develop an interpretive framework. In addition to direct applications to improved MALDI analysis, applications to understanding such surface properties as contact adsorption and biofouling are envisioned. The educational aspect of the proposal focuses on the development of laboratory courses and mentoring programs under the auspices of a Materials Science program at the institution.The laser-based method of mass spectrometric analysis depends on proper sample preparation. The work described by Gary Kinsel will establish the types of interactions between metal surfaces and the protein biomolecules often analyzed by laser-based mass spectrometry, and provide the information needed for sample preparation leading to higher sensitivity and specificity in analysis.