SGER: MALDI MS Analysis of Roughness Effects on Protein Binding to Pulsed Plasma Modified Polymer Surfaces

SGER：MALDI MS 分析粗糙度对脉冲等离子体改性聚合物表面蛋白质结合的影响

• 批准号: 9812708
• 负责人: Gary Kinsel
• 金额: $ 2.06万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 1999-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9812708&HistoricalAwards=false
• 关键词: SGER; MALDI; MS; Analysis; Roughness

9812708 Kinsel The goal of this proposed research is to study a new analytical approach to examine the dynamics of surface- biomolecule interactions. This approach is that of Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometry (MS). This technique could enable the examination of biomolecule surface adsorption at less than monolayer coverage. The problem to be examined centers on the effect of surface roughness in attenuating MALDI MS ion signals. Essentially, an inverse relationship has been observed in which, given a constant amount of biomolecule applied to a given polymer surface, the biomolecule MALDI ion signal increases in intensity as the surface-biomolecule binding affinity decreases. These studies, together with studies of the effect of changes in various surface properties on biomolecule adsorption and studies of the time dependence of biomolecule adsorption, promise to provide new insights into the fundamental processes which influence surface-biomolecule interactions. ***

9812708 Kinsel这项研究的目的是研究一种新的分析方法来检查表面-生物分子相互作用的动力学。 该方法是基质辅助激光解吸电离（MALDI）质谱法（MS）。 这种技术可以使生物分子的表面吸附在小于单层覆盖检查。 要研究的问题集中在衰减MALDI MS离子信号的表面粗糙度的影响。 本质上，已经观察到一种相反的关系，其中，给定施加到给定聚合物表面的恒定量的生物分子，随着表面-生物分子结合亲和力的降低，生物分子MALDI离子信号的强度增加。 这些研究，再加上生物分子吸附的各种表面性质的变化和生物分子吸附的时间依赖性的研究的影响的研究，承诺提供新的见解的基本过程，影响表面-生物分子相互作用。 ***