RF Plasma Polymer Modified MALDI Targets: Advanced Platforms for Biomarker Screening / Comparative Proteomics Applications

RF 等离子体聚合物修饰的 MALDI 靶标：生物标志物筛选/比较蛋白质组学应用的先进平台

• 批准号: 0719426
• 负责人: Gary Kinsel
• 金额: $ 35万
• 依托单位: Southern Illinois University at Carbondale
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-15 至 2011-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0719426&HistoricalAwards=false
• 关键词: RF; Plasma; Polymer; Modified; MALDI

Professor Gary Kinsel at Southern Illinois University in Carbondale is supported by the Analytical and Surface Chemistry Program to develop a novel type of sample target for use in biological matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The research explores the use of a pulsed radio frequency (rf) plasma to deposit polymer thin films on the surface of MALDI sample targets. Surface modification aims to enable acquisition of more mass spectral information about components of complex biological mixtures by utilizing the chemical properties of the polymer films to rapidly fractionate the peptides and/or proteins prior to MALDI MS analysis. These novel sample targets are expected to have important applications in disease diagnosis, bacterial fingerprinting, biomarker screening and biomedical research.The research engages post-doctoral, graduate, and undergraduate students in collaborative, interdisciplinary research. Collaborations involve application of the modified MALDI targets in cyanobacteria proteomics; novel surface-polymer grafting approaches to increase the peptide and/or protein binding capacity of the MALDI sample targets; and application of these multifunctional MALDI targets in breast cancer research.

南伊利诺伊大学卡本代尔分校的加里金塞尔教授得到了分析和表面化学项目的支持，开发了一种新型的样品靶，用于生物基质辅助激光解吸/电离（MALDI）质谱（MS）。 该研究探讨了使用脉冲射频（rf）等离子体存款聚合物薄膜的表面上的MALDI样品目标。 表面改性的目的是能够获得更多的质谱信息的复杂的生物混合物的成分，通过利用聚合物膜的化学性质，以快速破碎的肽和/或蛋白质之前，MALDI MS分析。 这些新的样品靶点有望在疾病诊断、细菌指纹图谱、生物标志物筛选和生物医学研究中有重要应用。该研究吸引了博士后、研究生和本科生进行跨学科的合作研究。 合作涉及在蓝细菌蛋白质组学中应用修饰的MALDI靶标;增加MALDI样品靶标的肽和/或蛋白质结合能力的新型表面聚合物接枝方法;以及这些多功能MALDI靶标在乳腺癌研究中的应用。