Activity Dependent Expression of Na+/K+ Pump Isoforms in Dorsal Root Ganglia Neurons

背根神经节神经元Na ~+/K ~+泵亚型的活性依赖性表达 (PI Dobretsov M.，神经元以电信号序列的形式传递信息.信息的形式由神经元连接决定，而信息本身则由序列中信号的数量和频率编码。 神经元面临的问题之一是，每个信号的传输都伴随着Na+在细胞中的积累。如果不将其从神经元中去除，这种积累的Na+可能会影响整个信号序列和整个信息本身的特性。 因此，神经元的功能似乎强烈依赖于Na+/K+泵的活性，Na +/K+泵是在动物细胞内维持Na+和K+浓度稳定的主要离子转运蛋白。此外，Na+/K+泵的活性和/或特性在具有不同放电强度和细胞内Na+控制的不同需要的神经元中应该是不同的。然而，除了理论上的考虑，很少有人知道神经元信号传导和钠流入神经元，亚型表达和神经元Na+/K+泵的特性之间存在的实际关系。 这些问题将在本研究中得到解决，将使用大鼠外周神经元作为神经元模型，电生理，组织学和荧光成像技术作为工具来表征神经元功能和Na+/K+泵的表达。

Activity dependent expression of Na+/K+ pump isoforms in dorsal root ganglia neurons. (PI Dobretsov M., NSF proposal # 9904815)Lay AbstractNeurons communicate information as sequences of electrical signals. The modality of the message is determined by the neuronal connections, while the message itself is coded by the number and frequency of signals within the sequence. One of the problems faced by neurons is that the transmission of each signal is followed by an accumulation of Na+ in the cell. If it is not removed from the neuron this accumulated Na+ may affect characteristics of the entire sequence of signals and of the whole message itself. It appears, therefore, that neuronal function should strongly depend on the activity of the Na+/K+ pump, the ion transporter that has a major role in maintaining stable concentrations of Na+ and K+ inside animal cells. Furthermore, it appears that the activity and/or characteristics of the Na+/K+ pump should differ in neurons with different discharge intensity and different needs in intracellular Na+ control. However, aside from theoretical considerations, little is known about actual relationship existing between the neuron signaling and sodium influx into the neuron, isoform expression, and characteristics of the neuronal Na+/K+ pump. These questions will be addressed in the present investigation that will use the rat peripheral neurons as a neuronal model, and electrophysiological, histological, and fluorescent imaging techniques as tools to characterize neuronal function and expression of the Na+/K+ pump.