SGER: System for Multidimensional Proteome Analysis

在完成基因组测序之后，下一个挑战将是描述特定细胞类型在特定情况下合成的蛋白质库，即蛋白质组学分析。这样的研究将需要能够处理非常复杂的蛋白质混合物的分析方法的发展。所建议的工作是针对这种方法的发展，使用集成的微流体（微芯片）系统，采用三种正交分离/分析模式（等电聚焦，分区色谱和质谱），然后对所得数据进行计算机分析。该系统将能够识别复杂混合物中的蛋白质，包括翻译后修饰的蛋白质和蛋白质混合物之间定量差异的定义。该系统的原型将使用最近开发的软光刻技术来实现。

9906662Abstract Following the complete sequencing of the genome, the next challenge will be to describe the repertoire of proteins synthesized by specific cell types under specific circumstances, i.e. proteome analysis. Such study will require development of analytical methodology capable of addressing very complex mixtures of proteins. The proposed work is directed toward development of such methodology using an integrated microfluidics (microchip) system employing three orthogonal separation/analysis modes (isoelectric focusing, partition chromatography and mass spectrometry) followed by computer analysis of the resulting data. The proposed system will be capable of identification of proteins in complex mixtures, including post-translationally modified proteins and definition of quantitative differences between protein mixtures. The prototype of the proposed system will be implemented using recently developed soft-lithography techniques.