Microbiological and Biochemical Analysis of Phosphorus Accumulating Organisms in Full Scale Wastewater Treatment Plants

[9909103] Noguera在该奖项的支持下进行的研究的目的是调查全面污水处理厂中磷酸盐去除的微生物学和生物化学，作为改进生物处理过程操作的步骤，以增强废水中磷酸盐的去除。实验室工作涉及应用一种技术，该技术基于微生物细胞之间的浮力密度差异，这些微生物细胞可以积累磷酸盐，而不可以积累磷酸盐，作为分离和隔离磷酸盐的物理方法。在这项工作中，一种程序将用于可视化多磷酸盐染色与荧光杂交相结合，以允许同时鉴定微生物和测定其聚磷酸盐的积累。实验室规模的研究结果将与从位于威斯康星州麦迪逊、斯托顿和拉辛的污水处理厂的微生物磷积累群落的研究中获得的知识相关联。麦迪逊的污水处理厂采用改良的UCT（开普敦大学）生物处理工艺，斯托顿工厂采用高级氧化工艺，拉辛污水处理厂采用传统的悬浮微生物生长、非强化生物除磷工艺。这项研究的结果有望将所获得的知识与利用微生物代谢活动从废水中去除植物营养物质的处理厂和系统的工程设计和操作的改进联系起来。这项工作是对美国国家科学基金会（NSF）资助号9875642的研究的补充，该研究的目的是研究曝气-缺氧工艺去除废水中氮的微生物生态。* * *

9909103 Noguera The objective of research being conducted with support by this award is to investigate the microbiology and biochemistry of phosphate removal in full-scale wastewater treatment plants as steps toward improvement in operation of biotreatment processes for enhanced removal of phosphates from wastewater. Laboratory work involves application of a technique based on differences of buoyant densities between cells of microorganisms that accumulate phosphates from those that do not accumulate them as a physical method for their separation and isolation. In this work, a procedure will be used for visualization of polyphosphate staining in combination with fluorescent hybridization to allow simultaneous identification of microorganisms and determination of their accumulations of polyphosphates. Results of laboratory-scale research will be correlated with knowledge obtained from studies of the microbial phosphorus accumulating communities of wastewater treatment plants located in Madison, Stoughton and Racine, Wisconsin. the wastewater treatment plant in Madison uses a modified UCT (University of Cape Town) biotreatment process, the Stoughton plant operates an advanced oxidation process and the Racine wastewater treatment plant uses a conventional suspended microbial growth, non-enhanced biological phosphate removal process. Results of this research are expect to relate knowledge obtained to improvement in the engineering design and operation of treatment plants and systems that utilize metabolic activities of microorganisms to remove plant nutrients from wastewater. This work is complementary to research being conducted with support from NSF Grant No. 9875642, the objective of which is to investigate the microbial ecology of nitrogen removal from wastewater by aerated-anoxic processes. ***