RUI: Cation-Chloride Cotransporters in Gills of the Fiddler Crab, Uca sp.

许多螃蟹能够在盐度波动很大的环境中生存，尽管这种能力即使在给定的属中也有很大的差异。渗透调节，当外部盐度波动时保持内部盐度恒定，需要将盐输送到体内或体外。盐转运发生在特殊的细胞层上，由盐转运蛋白介导。这项工作将侧重于开发一种针对无脊椎盐（Na-K-Cl）共转运体的抗体，并将证明在招潮蟹中检测Na-K-Cl共转运体的能力。开发针对招潮蟹共转运蛋白的物种特异性抗体需要克隆每个物种的转运蛋白。蓝蟹Na-K-Cl共转运体的全长序列现已进入序列数据库。这个来自相关生物的序列将有助于克隆工作。初步的证据将被收集来证明无脊椎动物阳离子-氯共转运体可以成功地表达和表征使用Sf9细胞表达系统。积累的数据将显示盐度胁迫后鳃蛋白的表达模式发生变化。特别是，这些分析将集中在证明前鳃和后鳃之间的差异。

Many crabs are able to survive in environments where salinity fluctuates widely, although this ability varies substantially even within a given genus. Osmoregulation, maintaining a constant internal salinity when external salinity fluctuates, requires transport of salt into or out of the body. Salt transport occurs across specialized cell layers and is mediated by salt transport proteins.This work will focus on development of an antibody against invertebrate salt (Na-K-Cl) cotransporters and will demonstrate the ability to detect Na-K-Cl cotransporters in fiddler crabs. Developing species-specific antibodies against fiddler crab cotransporters will require cloning transporters from each species. The full-length sequence of the blue crab Na-K-Cl cotransporter is now in the sequence database. This sequence from a related organism will facilitate the cloning efforts. Preliminary evidence will be collected to demonstrate that invertebrate cation-chloride cotransporters can be successfully expressed and characterized using the Sf9 cell expression system. Data will be accumulated to show that there are changing expression patterns in gill proteins after salinity stress. In particular, these analyses will focus on demonstrating differences between anterior and posterior gills.