SGER: Crystallization of a P1-Type Heavy Metal Transport ATPase

9985771 Arguelloon利用来自ATP的新陈代谢能量在活体的细胞膜上运输。转运蛋白是嵌入在膜中的酶，被归类为P型ATPase。对这些酶所执行的过程没有完全的了解，部分原因是缺乏对它们的原子结构的知识。最近几年，在原核生物中发现了一组新的P型ATPase(P1型)，它们可以运输重金属如铜、铜、锌、镉等进入细胞。这些酶具有与其他P型ATPase相同的机制特征，但它们的尺寸较小。这一特性使它们更容易分离、提纯、结晶和获得原子结构。通过对这一亚类P型ATPase的结晶学分析获得详细的结构信息，将为了解其他P型ATPase的离子选择性和能量转导机制以及更清楚地了解能量耦合离子传输提供信息。这一点尤其重要，因为这一知识还可以为有效利用微生物进行生物修复提供工程基础。

9985771ArguelloIons are transported across membranes of living organisms using metabolic energy derived from ATP. The transport proteins are enzymes that are imbedded in the membrane and classified as P-type ATPases. There is not a complete understanding of the processes performed by these enzymes due in part to a lack of knowledge of their atomic structures. During the last few years, a new group of P-type ATPases (P1-type) has been identified in prokaryotes that transport heavy metals such as Cu+1, Cu+2, Zn+2, Cd+2, and others into cells. These enzymes have the same mechanistic characteristics as other P-type ATPases, but they are smaller in size. This characteristic makes them easier to isolate, purify, crystallize and obtain their atomic structure. The detailed structural information obtained through crystallographic analysis of this sub-class of P-type ATPases will provide information on the ion selectivity and energy transduction mechanisms of other P-type ATPases and a clearer understanding of energy coupled ion transport. This is especially important since this knowledge may also provide a basis for the engineering of microorganisms that can be used effectively in bioremediation.