POWRE: Electronic Biosensors: An Integration of Nanotechnology with Molecular Biology

该POWRE奖的重点是PI获得新的实验技能和广泛的分子生物学知识。 PI建议通过在她的合作者D. A. notterman，普林斯顿大学分子生物学系。 在那里，她将集中精力将纳米技术与分子生物学相结合。 具体来说，她将开发一种集成微芯片，使用微流体通道和纳米电极的组合，可以测量单细胞的介电特性。 这些介电性质和细胞的DNA含量之间的相关性将被检查。 由于DNA含量分析和相关测量，如S期分数的测定，是检测癌细胞的潜在有用的策略，所提出的设备将是癌细胞的“芯片上”检测器。 PI将学习如何培养和操纵细胞。

The focus of the proposed research of this POWRE award is for the PI to acquire new experimental skills and a broad-based knowledge of molecular biology. The PI proposes to accomplish this by working in the laboratory of her collaborator, Dr. D. A. notterman, in the Department of Molecular Biology, Princeton University. There, she will focus her efforts toward integrating nanotechnology with molecular biology. Specifically, she will be developing an integrated microchip which, using a combination of microfluidic channels and nanoelectrodes, can measure the dielectric properties of single cells. Correlations between these dielectric properties and the DNA content of cells will be examined. Since DNA-content analysis and related measurements, such as the determination of S-phase fraction, are potentially useful strategies for detecting cancer cells, the proposed device will be an "on-chip" detector of cancer cells. The PI will be learning how to culture and manipulate cells for this project.