LTER Cross-site: The Relative Importance of Organic Material vs Local Environmental Conditions on Microbial Decomposer Communities: A Cross-site Comparison

米尔斯，亚伦L哥伦比亚大学DEB 0087256”有机物质与当地环境条件对微生物分解者群落的相对重要性：跨站点比较“这项工作将解决植物凋落物与当地环境条件在确定分解者群落组成方面的相对重要性问题。 将使用来自10个大西洋河口站点的社区，包括4个LTER站点（弗吉尼亚海岸保护区、格鲁吉亚沿海站点、梅岛河口、佛罗里达大沼泽地）和其他6个研究站点。 将比较三种类型的植物凋落物（无芒灯心草、红红树林和当地优势的高沼泽植物物种），以测试当地环境对该植物物种的分解者群落的影响。 在分解过程中，将在1、2、6、12和18个月时收集样品，并将分子和培养技术相结合，以评估微生物群落。 将培养物冷冻并储存为文库，可根据需要进行访问和检查，以比较微生物群落可培养部分的组成与分子方法获得的组成。

Mills, Aaron LUniversity of VirginiaDEB 0087256" The relative importance of organic material vs. local environmental conditions on microbial decomposer communities: a cross-site comparison"This work will address the issue of the relative importance of plant litter vs. local environmental conditions in determining the community composition of decomposers. Communities from 10 Atlantic estuarine sites, including four LTER sites (the Virginia Coast Reserve, Georgia Coastal site, Plum Island Estuary, Florida Everglades) and six other research sites will be used. Three types of plant litter (smooth cordgrass, red mangrove, and the locally dominant high marsh plant species) will be compared to test the effect of local environment on the decomposer community for that plant species. During decomposition, samples will be collected at 1, 2, 6, 12, and 18 months and a combination of molecular and culture techniques will be done to assess microbial communities. Cultures will be frozen and stored as a library that can be accessed and examined as needed to compare the composition of the culturable fraction of the microbial community with that obtained with the molecular methods.