Neurobiology Laboratory Experience for Biology Majors and Other Advanced Students

生物科学(61)本项目通过使用实验神经生物学实验室部分的计算机化工作站来加强基础科学课程。实验神经生物学是围绕实验室的组成部分而构建的，并提供了一种深入研究的方法。学生学习现代神经生物学技术，包括：细胞内记录、细胞外记录、动作电位识别、神经元束追踪、神经元染色和成像。这些技术是通过无脊椎动物模型准备学习的，这些准备工作允许新手学生成功地记录神经系统，并在每周三个小时的时间内进行实验。许多实验室课程都是根据美国国家科学基金会资助的Crawda项目进行的调查改编而成的，我们正在开发几项基于其他无脊椎动物的新活动。每个学生设计并进行为期四周的独立调查，最后以口头陈述和一个网页说明他们的发现。这些工作站还使其他五门生理学/细胞生物学课程的实验室活动受益。此外，这种设备极大地扩大了本科生获得神经科学独立研究经验的机会。最后，该设备还使我们能够将神经科学实验室体验整合到两门职前教师所需的科学教育课程中。

Biological Sciences (61) This project is strengthening the basic science curriculum through use of computerized work stations in the laboratory component of Experimental Neurobiology. Experimental Neurobiology is structured around the laboratory component, and provides a heavily investigative approach. Students learn modern neurobiology techniques including: intracellular recording, extracellular recording, action potential discrimination, neuronal tract tracing, and neuron staining and imaging. These techniques are learned using model invertebrate preparations that allow novice students to successfully record from nervous systems and perform experiments in a three-hour period each week. Many laboratory sessions are adaptations of investigations from the NSF-funded Project Crawdad, and we are developing several new activities based on other invertebrates. Each student designs and carries out an independent four-week investigation culminating in an oral presentation and a web page illustrating their findings. The work stations are also benefiting the laboratory activities in five other physiology/cell biology courses. Also, this equipment is greatly expanding opportunities for undergraduates to gain an independent research experience in neuroscience. Finally, the equipment is also allowing us to integrate a neuroscience laboratory experience in two science education courses required for pre-service teachers.