Molecular Biology Course Based on Study of Transcriptional Control

生物科学（61）本项目的目的是建立一个基础分子生物学实验室，在分子生物学新课程的背景下，该实验室将在课程中实施研究性实验。该项目旨在将美国国家科学基金会支持的“酵母实验课堂指南”改编为一个有趣的，未知的研究问题，作为分子生物学学生的起点。实验方法为学生提供关键分子生物学协议的实践训练，这些协议应用于有关转录控制和重要分子的进化保护的研究问题。分子生物学实验室的设计方式使学生能够在三个基本水平上分析基因，即DNA， RNA和蛋白质，使用体外和体内方法，并产生学生的学习成果。本课程强调理解方法、分子方法和仪器的重要性，这些方法彻底改变了生物学，是疾病诊断和预防的关键，同时也允许学生讨论这些新技术所带来的伦理考虑。购置的设备也用于改进其他生物课程的实验室部分，即遗传学、细胞生理学和生物化学，从而使乌格罗格大学的生物课程现代化。特定设备，如聚合酶链反应热循环仪和凝胶电泳仪，正在用于作为普通教育要求提供的非主要人类生物学课程，以展示分子生物学在法医科学、亲子案件、人类学和考古学调查中的应用。

Biological Sciences (61) The aim of this project is to establish a basic molecular biology lab that, in the context of a new course in Molecular Biology, will implement research-based experiments into the curriculum. The project intends to adapt the NSF-supported "Classroom Guide to Yeast Experiments" to an interesting, unknown research question that serves as a starting point for molecular biology students. The experimental approaches provide students with hands-on training in key molecular biology protocols that are applied to a research problem regarding transcriptional control and the evolutionary conservation of important molecules. The Molecular Biology lab is designed in a manner that enables students to analyze genes at three fundamental levels, namely, DNA, RNA, and Proteins, using both in vitro and in vivo approaches, as well as produce student learning outcomes. The course emphasizes the importance of understanding methodologies, molecular approaches, and instrumentation that have revolutionized biology and are key to diagnosis and prevention of disease, as well as allow the students to debate the ethical considerations that some of these new technologies pose. The equipment being acquired is also being used to improve the laboratory component of other biology courses, namely Genetics, Cell Physiology, and Biochemistry, thereby modernizing the Biology curriculum at UOG. Specific equipment like the PCR Thermal Cycler and gel electrophoresis apparatus is being used for the non-major Human Biology course offered as a general education requirement, in order to demonstrate applications of molecular biology in forensic sciences, paternity cases, anthropological and archaeological investigations.