The Molluscan Mitochondrial Genomic Project: Phylogenetic Analysis, Evaluation and Enrichment

DEB-0089624大卫河林德伯格博士大卫河位于伯克利的加州大学的林德伯格获得了一笔资金，用于对50种腹足类软体动物的线粒体基因组进行测序。 虽然腹足类的进化历史已经被研究了很长时间，并且该组有很好的化石记录，但由于形态趋同和先前分析的基因的无信息性，腹足类树底部的分支模式知之甚少。 该项目将研究线粒体基因顺序，这是一个有前途的新数据集，用于软体动物，并使用这些数据来重建腹足类内以及腹足类与其他软体动物之间的关系。 在过去，线粒体基因组的完整测序是如此耗时，以至于很少有软体动物被研究。 然而，随着几个主要测序计划的完成，高通量测序设施变得可用。 该项目将使用劳伦斯伯克利国家实验室的高通量果蝇基因组测序中心，以及美国，德国和澳大利亚研究人员合作分析的数据。 该项目的具体目标包括鉴定、分析和评估线粒体基因组中的基因、rRNA和tRNA位置、二级结构和重建关系的序列。 化石记录也将被用来关联第一次出现的数据，并评估每个基因在不同的腹足类群体的分辨率潜力，并提供重要的见解macroevolutionary模式，目前的建议，但我们目前缺乏了解最早的腹足类软体动物之间的关系的限制。 它还将为无数无脊椎动物学家、分子生物学家、生物信息学家和分子进化学家提供初步和探索性数据，并将作为一个试点项目，评估与劳伦斯伯克利和其他国家实验室等高通量测序中心的合作伙伴关系，并探索它们在重建生命之树中的潜在作用。

DEB-0089624David R. LindbergDr. David R. Lindberg at the University of California at Berkeley has received funding to sequence complete mitochondrial genomes of 50 species of gastropod mollusks. Although the evolutionary history of gastropods has been long studied and the group has an excellent fossil record, the branching patterns at the base of the gastropod tree are poorly understood because of morphological convergence and the uninformative nature of the genes that have been previously analyzed. This project will examine mitochondrial gene order, a promising new data set for mollusks, and use these data to reconstruct the relationships within the Gastropoda and between gastropods and other mollusks. In the past complete sequencing of mitochondrial genomes has been so time consuming that few mollusks have been studied. However, with the completion of several major sequencing initiatives, high-throughput sequencing facilities are becoming available. This project will use the high-throughput Drosophila Genome Sequencing Center at Lawrence Berkeley National Laboratory, and the data analyzed by a collaboration of researchers in the USA, Germany and Australia. Specific aims of this project include the identification, analysis and evaluation of gene, rRNA, and tRNA placement within the mitochondrial genome, secondary structures, and sequences for reconstructing relationships. The fossil record will also be used to correlate first appearance data and evaluate the resolution potential of each gene in different gastropod groups, and provide important insights into macroevolutionary patterns that are currently suggestive, but limited by our current lack of understanding of the relationships among the earliest gastropod molluscs. It will also provide preliminary and exploratory data for countless invertebrate zoologists, molecular biologists, bioinformaticists, and molecular evolutionists, and will serve as a pilot project for the evaluation of partnerships with high-throughput sequencing centers such as those housed at Lawrence Berkeley and other National Laboratories, and explores their potential role in reconstructing the Tree of Life.