Differential, dynamic syndapin complexes - modulators of membrane topology and transport

真核细胞周围的质膜将细胞内部与环境分开。膜拓扑调节对于膜运输过程中的囊泡形成以及不同细胞形态的建立、维持和可塑性是必不可少的，这是多细胞生物发育和生存的先决条件。它是由弯曲、管状和/或断裂膜的直接效应器的协调相互作用以及膜相关皮质细胞骨架的组织和动力学催化的。BAR结构域超家族蛋白，如syndapin家族的F-BAR结构域蛋白，已成为膜形成的重要组成部分。该项目旨在提供进一步的证据，证明syndapins具有将其新月形印记在膜上的能力，并进一步招募、协调和调节细胞效应物的活动，对于形成细胞膜和细胞器以及细胞结构和整个生物体的发育是必不可少的。该工作计划的成功实现有望揭示syndapin介导的膜拓扑控制如何允许形成细胞膜，并揭示膜形成损伤对单个细胞膜区室，不同细胞类型的形态和功能以及整个器官和生物体的正常功能的生理后果。

The plasma membrane, which surrounds eukaryotic cells, separates the cellular interior from the environment. Membrane topology modulation is indispensable for vesicle formation in membrane trafficking processes as well as for establishment, maintenance and plasticity of distinct cellular morphologies - prerequisites for development and live of multicellular organisms. It is catalyzed by the coordinated interplay of direct effectors that bend, tubulate and/or scission membranes and by organization and dynamics of the membrane-associated cortical cytoskeleton. BAR domain superfamily proteins, such as the F-BAR domain proteins of the syndapin family, have emerged as important components in membrane shaping. The project aims at providing further evidence that syndapins, which have the power to imprint their crescent shape onto membranes and furthermore recruit, coordinate and modulate the activity of cellular effectors, are indispensable for shaping cellular membranes and organelles and for the development of cellular structures and whole organisms. The successful realization of the work program is expected to unravel how syndapin-mediated membrane topology control allows for shaping cellular membranes and to reveal the physiological consequences of membrane shaping impairments on individual cellular membrane compartments, on the morphology and function of distinct cell types and on proper function of entire organs and organisms.