A Wide-Field Imaging System for Living Cells

该合同为购买一种宽视场共聚焦显微镜提供了资金，该显微镜配备了两个高灵敏度、增强电荷耦合器件相机、一个旋转盘型共聚焦成像设备和成像软件。该细胞系统能够高速、双激发或双发射比成像，并包括活细胞显微注射所需的设备。这台显微镜将成为发育动力学研究新实验室的核心设备，该实验室将由多名研究人员共享。高灵敏度探测器允许扩展荧光成像的发展系统，这是不可能与激光扫描共聚焦显微镜，或多光子仪器。该系统将被博士后助理、研究生和本科生以及教师研究者用于细胞生物学的各种研究项目，并将为现代光学显微镜技术的扩展课程提供基础。

This award provides funds for purchase of a wide field confocal microscope equipped with two high- sensitivity, intensified charge-coupled-device cameras, a spinning disk-type confocal imaging device, and imaging software. The cells system capable of high speed, dual excitation or dual emission ratiometric imaging, and includes equipment needed for microinjection of living cells. The microscope will be the centerpiece for a new Laboratory for the Study of Developmental Dynamics to be shared by a number of investigators. The high-sensitivity detectors allow extended fluorescence imaging of developing systems that is not possible with laser scanning confocal microscopes, or multi-photon instruments. The system will be used by postdoctoral associates, graduate and undergraduate students, and faculty investigators for a variety of research projects in cell biology, and will provide the basis for an expanded course on modern techniques in optical microscopy.