Glucosinolate hydrolysis in plants - the role of specifier proteins

芥子油苷-芥子酶系统是十字花科植物及其相关科植物的活性化学防御系统，其多样性源于芥子油苷生物合成和芥子油苷水解的差异。根据硫代葡萄糖苷的结构和某些蛋白质因子、指示蛋白的存在，一种硫代葡萄糖苷可以被水解成具有不同生物活性的不同产物。我们项目的目标是学习理解，如何以及为什么植物在硫代葡萄糖苷水解水平上产生多样性。本项目旨在探索拟南芥中特异蛋白的生物学作用、调控和定位，通过研究特异蛋白的结构及其与黑芥子酶的相互作用来阐明特异蛋白的生化机制，并研究该蛋白家族在含硫代葡萄糖苷植物中的进化过程。

The diversity of the glucosinolate-myrosinase system, an activated chemical defense of the Brassicaceae and related families, arises from the variation of both glucosinolate biosynthesis and glucosinolate hydrolysis. Depending on the glucosinolate structure and the presence of certain protein factors, the specifier proteins, one glucosinolate can be hydrolyzed to different products with diverse biological activities. The goal of our project is to learn to understand, how and why plants generate diversity at the level of glucosinolate hydrolysis. The proposed project is aimed at exploring the biological role, regulation and localization of specifier proteins in A. thaliana, at elucidating the biochemical mechanism of specifier proteins based on the investigation of their structure and their interaction with myrosinase, and at studying how the protein family has evolved in glucosinolate-containing plants.