Genetic Analysis of Tobamovirus-Host Interactions

简单的RNA病毒已经进化出与其宿主的细胞机制的复杂相互作用，以促进其复制、细胞间运动和系统转运。 这些相互作用通常导致宿主细胞过程的发育和稳态的破坏，导致影响宿主健康和/或其经济价值的疾病症状的表达。 该项目的目标集中在识别参与病毒复制或受病毒复制影响的宿主组件。为了实现这些目标，植物病毒烟草花叶病毒（TMV）的分子工具将与拟南芥的遗传可塑性相结合，以确定新的宿主基因，直接有助于病毒的生命周期或在感染过程中被转录改变。 本项目的具体研究将涉及三个目标。 1)基于图位的克隆方法将被用来确定一个先前分类的拟南芥基因座，控制TMV细胞到细胞的运动速度。 进一步的研究将解决这个位点的功能及其在病毒细胞间运动中的作用。 2)可用的微阵列技术将被用来确定拟南芥基因的差异调节过程中的TMV感染周期的不同阶段，包括接种和全身感染的全叶组织，以及同步感染的原生质体。 3)由TMV感染的拟南芥表现出的症状将用于鉴定具有改变的病毒亲和性的拟南芥突变体。 然后将启动基于图位的克隆策略，以确定赋予这些改变的亲合性的基因。 总的来说，该项目的研究应该大大增强我们对病毒与宿主之间相互作用的理解。

Simple RNA viruses have evolved complex interactions with their host's cellular machinery that facilitate their replication, cell-to-cell movement, and systemic transport. These interactions often lead to a disruption in the development and homeostasis of the host's cellular processes, resulting in the expression of disease symptoms that impact the host's well being and/or its economic value. The goals of this project focus on the identification of host components that are either involved in or affected by virus replication. To accomplish these goals the molecular tools available for the plant virus Tobacco mosaic virus (TMV) will be combined with the genetic tractability of Arabidopsis thaliana to identify novel host genes that either directly contribute to the virus life-cycle or are transcriptionally altered during the infection process. Specific studies in this project will address three objectives. 1) A map-based cloning approach will be used to identify a previously classified Arabidopsis locus that controls the rate of TMV cell-to-cell movement. Additional studies will address the function of this locus and its role in virus cell-to-cell movement. 2) Available microarray technologies will be used to identify Arabidopsis genes that are differentially regulated during distinct stages of the TMV infection cycle, including inoculated and systemically infected whole leaf tissue as well as synchronously infected protoplasts. 3) Symptoms displayed by TMV infected Arabidopsis will be used to identify Arabidopsis mutants with altered virus susceptibilities. Map-based cloning strategies will then be initiated to identify the genes that impart these altered susceptibilities. Overall, studies from this project should significantly enhance our understanding of the interactions that occur between viruses and their hosts.