Light-responsive cis-elements in nuclear genes

为了进一步了解植物细胞中光调控基因的表达以及核基因表达与光合作用的协调，拟南芥增强诱捕株系（eTrap株系）将筛选光敏顺式调控元件。在分析的早期阶段，将根据体内报告基因监测将增强子元件细分为光强、光质量或光合作用调节元件。通过热不对称交叉PCR （TAIL-PCR）鉴定顺式调控元件和调控基因。酵母-混合筛选将与增强子元件一起进行，以筛选上游反式调节因子。最后，鉴定出的调节蛋白将被表征其在拟南芥过表达、沉默和敲除系中的功能。将特别关注叶绿体发育、光合作用和叶绿体-核协调的影响。

To improve our understanding of light regulated gene expression in plant cells and of coordination of nuclear gene expression with photosynthesis, Arabidopsis thaliana enhancer trap lines (eTrap lines) will be screened for light-sensitive cis-regulatory elements. At an early step of the analysis, the enhancer elements will be sub-classified based on in vivo reporter gene monitoring into light intensity, light quality or photosynthetically regulated ones. The cis-regulatory elements and the regulated genes will be identified by thermal asymmetric interlaced PCR (TAIL-PCR). Yeast-one-hybrid screens will be performed with the enhancer elements to screen for upstream trans-regulators. Finally, the identified regulatory proteins will be characterized for their function in Arabidopsis over-expressor, silencer and knock-out lines. Special attention will be given to the impact of chloroplast development, photosynthesis and chloroplast-nucleus coordination.