BAC Library: Diversity of Teleost Fishes

0208343 KocherTeleost鱼类是世界各地水生生态系统的主要组成部分，并且作为动物蛋白的主要来源具有巨大的商业重要性。 硬骨鱼类在过去的200MY中经历了一场壮观的适应性辐射。 25，000种硬骨鱼占所有现存脊椎动物分类群的一半以上。鱼类是从生态学、进化和发展到生物医学的生物学研究领域的重要模型系统。为人类基因组测序而开发的基因组工具现在正在许多分类群中找到应用。 目前正在对三种鱼类（Danio、Fugu和Tetraodon）的基因组进行全面测序。已经为其他十几个物种绘制了遗传图谱，其中一些物种的物理图谱正在绘制中。 其他物种的BAC文库将进一步研究，旨在发现物种之间的系统发育关系，分类群之间适应性差异的遗传基础，以及调节基因组进化的模式和过程。该项目旨在通过构建和分发代表主要硬骨鱼谱系的两个物种的大插入基因组DNA（BAC）文库，进一步促进鱼类的比较基因组研究。它们是盲洞穴鱼（Astyanax mexicanus）和马拉维湖斑马（Metriaclima zebra）。 这些BAC文库将通过UNH Hubbard基因组研究中心永久存档和分发，该中心是一个专注于比较和环境基因组学的新基因组中心。 每个文库将作为克隆分布在384孔板和适于杂交筛选的网格过滤器中。广泛的质量控制措施将确保每个衍生产品（复制板或过滤器）可以追溯到原始克隆。哈伯德中心致力于鱼类比较基因组资源的开发。 这项建议是一个计划的第一步，以产生基因组和cDNA文库，遗传和物理图谱，比较映射数据库的鱼类。 这里提出的图书馆将作为未来研究的基础，在遗传学，基因组进化，发展，进化，环境生物学和水产养殖。

0208343KocherTeleost fishes are dominant components of aquatic ecosystems around the world, and are of tremendous commercial importance as a major source of animal protein. Teleosts have undergone a spectacular adaptive radiation over the last 200MY. The 25,000 species of teleost represent more than half of all living vertebrate taxa. Fishes are important model systems across the spectrum of biological research, from ecology, evolution and development to biomedicine. The genomic tools developed to sequence the human genome are now finding applications across many taxa. The genomes of three fish species (Danio, Fugu and Tetraodon) are being completely sequenced. Genetic maps have been constructed for a dozen other species, and physical maps are under construction for a few of these. BAC libraries for additional species will further research aimed at discovering the phylogenetic relationships among species, the genetic basis for adaptive differences among taxa, and the patterns and processes that regulate genome evolution. This project aims to further stimulate comparative genomic studies of fishes by constructing and distributing large-insert genomic DNA (BAC) libraries for two species representing major teleost lineages. They are the blind cavefish (Astyanax mexicanus), and the Lake Malawi zebra (Metriaclima zebra). These BAC libraries will permanently archived and distributed through the UNH Hubbard Center for Genome Studies, a new genome center focused on comparative and environmental genomics. Each library will be distributed as clones in 384-well plates and gridded filters suitable for hybridization screening. Extensive quality control measures will ensure that each derived product (replica plate or filter) can be traced back to the original clone. The Hubbard Center is committed to the development of comparative genomic resources for fishes. This proposal is the first step in a program to generate genomic and cDNA libraries, genetic and physical maps, and comparative mapping databases for fishes. The libraries proposed here will serve as a foundation for future research in phylogenetics, genome evolution, development, evolution, environmental biology and aquaculture.__