SBIR Phase I: Cavity-Enhanced Capillary Electrophoresis

这个小型企业创新研究第一阶段项目建议将腔增强光谱技术应用于毛细管电泳法(CE)。毛细管电泳法通常依靠吸收光谱来检测从分离柱中洗脱出来的分析物。虽然这种检测方法非常有用，但毛细管的短吸收路径长度限制了其灵敏度，迫使开发更复杂的检测方案。初步结果表明，可以使用光学腔体将这种吸收灵敏度提高几百到一万倍以上。该方案的目的是将CE柱连接到光腔，并展示对各种生物样品的增强检测。由于CE在生化研究中的日益普及，这项技术的商业应用也在不断增长。它目前被用于分析DNA片段、蛋白质和各种药物。此外，它还被认为是DNA测序的凝胶电泳法的替代品，是芯片实验室技术的领先前景。该项目的商业应用包括基因组测序、医疗诊断和药物筛选。

0211557GuptaThis Small Business Innovation Research Phase I Project proposes to apply cavity-enhanced spectroscopy to Capillary Electrophoresis (CE). CE typically relies on absorption spectroscopy to detect analytes eluting from a separation column. Although this detection method is very useful, the capillary's short absorptionpathlength has limited its sensitivity, forcing the development of more complex detection schemes. Preliminary results suggest that an optical cavity can be used to enhance this absorption sensitivity by a factor of hundred to over ten thousand. The aim of this proposal is to interface a CE column to an optical cavity and demonstrate the enhanced detection of various biological samples. Due to the increasing popularity of CE in biochemical research, the commercial applications of this technology continue to grow. It is currently used to analyze DNA fragments, proteins, and various drugs. Moreover, it is also being considered as an alternate to gel electrophoresis for DNA sequencing and is a leading prospect for lab-on-a-chip technologies. The commercial applications of this project are in genomic sequencing, medical diagnostics, and drug screening.