Functional Genomics of Host-Virus Interactions

真核病毒和它们的宿主之间的关系是共同进化的苔藓-病原体关系的特征。病毒-宿主相互作用的结果是遗传上预先决定的。为了制造疾病，病毒必须进入宿主，在宿主组织中局部繁殖，从进入部位传播，并克服或逃避宿主免疫反应。植物已经进化出各种抗病毒防御策略来清除病毒感染。另一方面，病毒也进化出了反防御策略。因此，了解病毒如何逃避宿主抗病毒防御的分子机制非常重要。基于烟草Rattle病毒的病毒诱导基因沉默(VIGS)系统将用于鉴定病毒抗性抑制因子和易感因子。此外，还将使用功能蛋白质组学方法来研究病毒与宿主之间的相互作用。从这些研究中获得的知识将有助于抗击传染性植物疾病。保护农作物免受疾病侵袭可以显著提高农业产量。应用植物自身的防御机制可以更有效地防御植物病原菌。利用在抗病信号通路中发挥作用的细胞基因来控制病原体引起的疾病，可能会提供巨大的农业效益，并通过提供一种替代农药的方法来服务于环境。将向科学界提供在该项目中开发的工具和信息。这些资源将有助于改进番茄、马铃薯和辣椒等重要经济作物。目前可提供：pTRV1、pTRV2、pTRV2-GATENT、pTRV-NbPDS、pTRV-TOMPDS VIGS系统。联系savithramma.dinesh-kumar@yale.edu9/03：250-300序列验证pTRV2-番茄EST克隆1000-1500序列验证pTRV2-NB-cDNA农杆菌TMV-TAP载体

The relationship between eukaryotic viruses and their hosts are characteristic of mosthost-pathogen relationships that have co-evolved. The outcomes of virus-host interactions are genetically pre-determined. To produce disease, viruses must enter the host, multiply locally in host tissues, spread from the site of entry, and overcome or evade host immune responses. Plants have evolved various anti-viral defense strategies to clear viral infection. On the other hand, viruses have evolved counter-defense strategies. Therefore, it is important to understand molecular mechanisms of how viruses evade the host's antiviral defenses.A Tobacco Rattle Virus-based virus induced gene silencing (VIGS) system will be used to identify suppressors of viral resistance and susceptibility factors. In addition, a functional proteomics approach will be used to study virus-host interactions. The knowledge gained from these studies will help to combat infectious plant diseases. Protection of crops from disease can significantly improve agricultural production. Application of a plant's own defense mechanism can lead to more effective protection against plant pathogens. Control of pathogen-induced diseases using cellular genes that function in the disease resistance signaling pathway may provide tremendous agricultural benefits and serve the environment by offering an alternative to pesticide use to prevent disease. Tools and information developed in this project will be made available to the scientific community. These resources will assist in the efforts to improve economically important plants like tomato, potato and pepper.DeliverablesAvailable now: pTRV1, pTRV2, pTRV2-GATEWAY, pTRV-NbPDS, pTRV-tomPDS VIGS system. Contact savithramma.dinesh-kumar@yale.eduAvailable by 9/03: 250-300 sequence verified pTRV2-tomato EST clones 1000-1500 sequence verified pTRV2-Nb-cDNA Agrobacterium TMV-TAP vector