Insertional Mutagenesis Tools for Functional Genomics in Rice

水稻是第二个被完全测序的植物基因组，其基因组相对较小，约为4.3亿个碱基对。 虽然水稻也适合遗传研究和转化，并且是谷物基因组研究的模型系统，但它也是一种重要的作物。 目前，有几个工具，用于确定水稻基因测序的功能，是很容易提供给研究界。 破译基因功能的最有效方法之一是通过DNA插入破坏基因。 在本计画中，我们将发展有效的转座子标签策略，以进行水稻的大规模插入突变。 将产生稳定的插入系，其含有在粳稻cv中随机插入的玉米En/Spm转座子。日本晴，这是国际水稻基因组测序计划选择的品种。 将建立一个可公开访问的反向遗传学数据库，其中包含DNA侧翼插入序列，这些序列将与水稻基因组数据库和水稻基因组物理图谱连接。 总的来说，该项目将为水稻和其他谷物的功能基因组学开发宝贵的资源。

Award AbstractRice, the second plant genome to be completely sequenced, has a relatively small genome of about 430 million base pairs. While rice is also amenable to genetic studies and transformation and is a model system for genome studies of the cereals, it is also an important crop. Currently, there are few tools for identifying the functions of sequenced genes for rice that are readily available to the research community. One of the most effective approaches to deciphering gene functions is through gene disruption by DNA insertions. In this project, we will develop efficient transposon tagging strategies for large-scale insertion mutagenesis in rice. Stable insertion lines will be generated containing random insertions of the maize En/Spm transposons in japonica rice cv. Nipponbare, which is the cultivar selected for the International Rice Genome Sequencing Project. A publicly accessible database for reverse genetics containing sequences of DNA flanking insertions will be established, and the sequences will be linked to the rice genome database and the rice genome physical map. Overall, this project should develop a valuable resource for functional genomics in rice, as well as other cereals.