Fluorescence Lifetime-Based Measurements of Biosensor Arrays Using Closed Loop Auto-Oscillating Systems

新墨西哥大学的加布里埃尔·洛佩兹教授由分析和表面化学计划资助，开发了一种基于激发态寿命的生物传感器阵列荧光检测方法。该方法基于发射光的相位信息，在灵敏度方面具有荧光的优势，比仅仅是波长特性具有更大的选择性。PI实验室开发的闭环自动振荡系统将在稳定性和可靠性方面进行改进，并将被内置到传感器平台中。学生们将学习生物传感、光学和仪器。这项小型探索性研究拨款的工作也将对光谱分析产生总体影响。检测痕量生物制剂的一种方法是检测试剂上的分子标签发出的光。坚固、可靠和廉价的灵敏探测器的开发将对我们感知和识别生物制剂的能力产生影响。这项研究具有国家安全影响以及潜在的医疗和环境应用。

Professor Gabriel Lopez of the University of New Mexico is funded by the Analytical and Surface Chemistry Program to develop a fluorescence detection method for biosensor arrays based on excited state lifetimes. The method, based on phase information about the emitted light, would have the advantages of fluorescence in terms of sensitivity with greater selectivity than just wavelength characteristics alone. The closed loop auto-oscillating system developed in the PI's lab will be improved for stability and reliability and will be built into a sensor platform. Students will learn biosensing, optics and instrumentation. The work in this Small Grant for Exploratory Research will also have impact in spectroanalysis in general.One approach to sensing trace biological agents is to detect light emitted from a molecular tag on the agents. The development of sensitive detectors that are rugged, reliable and inexpensive would have impact on our ability to sense and identify biological agents. This research has national security implications as well as potential medical and environmental applications.