Development of fiber-optic, fluorescence lifetime-based imaging system for in vivo measures of signalling events

开发基于荧光寿命的光纤成像系统，用于信号事件的体内测量

• 批准号: 515775-2017
• 负责人: Hébert, Terence
• 金额: $ 1.82万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639690
• 关键词: Development; fiber; optic; fluorescence; lifetime

The ability to image and record cellular processes in living animals represents one of the most powerfulapproaches in biological research. A variety of protein based optical indicators or "biosensors" have been usedto report cellular processes in cultured cells. However, imaging these biosensors in live animals hastraditionally been challenging, requiring costly transgenic animals and specialized microscopy equipment.These obstacles can be overcome with the recent development of affordable fiber-optic imaging systems,together with improved genetically-engineered biosensors and viral delivery methods. This Engage proposalaims to develop a novel fiber-optic photon counting system that would provide an affordable tool for recordingbiosensor emissions in live animals.

在活体动物中成像和记录细胞过程的能力代表了生物研究中最有力的方法之一。各种基于蛋白质的光学指示剂或“生物传感器”已被用于报告培养细胞中的细胞过程。然而，在活体动物中成像这些生物传感器一直具有挑战性，需要昂贵的转基因动物和专门的显微镜设备。这些障碍可以通过最近开发的负担得起的光纤成像系统，以及改进的基因工程生物传感器和病毒传递方法来克服。该Engage提案旨在开发一种新的光纤光子计数系统，该系统将为记录活体动物的生物传感器排放提供负担得起的工具。