US-France Cooperative Research: Evolution and Function of Hopanoid Lipids in Symbiotic and Thermoacidophilic Actinomycetes

0233366贝里这个为期三年的奖项，为美国和法国在微生物遗传学合作涉及艾莉森M。加州大学戴维斯分校的贝瑞和克劳德·伯纳德-里昂大学的菲利普·诺曼德1和他们的学生。 目标是研究Frankia的生物学，Frankia是一种形成根瘤共生的固氮原核生物，并解决一个关键的代谢控制点，hopanoid脂质生物合成途径。 Frankia是一种重要的微生物，它能产生特定的类藿烷脂类，这与革兰氏阴性根瘤菌类似，它们能产生一组相关的固氮植物。 Frankia与嗜热嗜酸纤维嗜酸菌（Acidothermus cellulyticus）有关。 他们将通过以下方式探索类Hopanoids在共生固氮中的作用以及作为对环境胁迫的适应：（1）来自17-kb Frankia基因组克隆的DNA序列信息，以及近亲中的相应基因：（2）Frankia hopanoid脂质与A.嗜热嗜酸放线菌的环境采样及hopanoid基因的表达。 在这次合作中获得的信息将用于在极端环境条件下和共生信号化合物的Hopanoid合成的进化和功能的扩展调查。美国研究人员带来了这项合作的专业知识，在hopanoid脂质及其重要性，固氮在Frankia。法国在Frankia plugeny和使用分子遗传工具检查土壤中微生物群落方面的专门知识对此起到了补充作用。 该小组也有能力对Frankia进行蛋白质组学分析。该奖项代表了美国方面向NSF和法国国家科学研究中心（CNRS）提出的联合建议。 NSF将支付美国研究员和研究生的旅费和生活费。 CNRS将支持法国研究人员和学生访问美国。 美国学生将受益于与国际研究团队的科学技术和经验交流。

0233366BerryThis three-year award for US-France collaboration in microbial genetics involves Alison M. Berry of the University of California at Davis and Philippe Normand of the University of Claude Bernard - Lyon 1 and their students. The objectives are to study the biology of Frankia, a nitrogen-fixing prokaryote that forms root nodule symbioses and to address a key metabolic control point, the hopanoid lipid biosynthetic pathway. The production of specific hopanoid lipids in Frankia is a pivotal microorganism in that it is similar to the gram-negative rhizobia in its capacity to nodulate a group of related nitrogen-fixing plants. Frankia is related to thermoacidophilic Acidothermus cellulyticus. They will explore the role of hopanoids in symbiotic nitrogen fixation and as an adaptation to environmental stress through (1) DNA sequence information from the 17-kb Frankia genomic clone, and corresponding genes in close relatives: (2) comparisons of the profile of Frankia hopanoid lipids with that of A. Celluloyticus; and (3) environmental sampling of the thermoacidophilic actinomycetes, and expression of hopanoid genes. The information obtained in this collaboration will be used in expanded investigations of the evolution and function of hopanoid synthesis under extreme environmental conditions and by symbiotic signaling compounds. The US investigator brings to this collaboration expertise in hopanoid lipids and their importance in nitrogen fixation in Frankia. This is complemented by French expertise in Frankia phylogeny and in the use of molecular genetic tools for examining microbial communities in soils. This group also has the capacity to do proteomic analysis of Frankia. This award represents the US side of a joint proposal to the NSF and the French National Center for Scientific Research (CNRS). NSF will cover travel funds and living expenses for the US investigator and graduate students. The CNRS will support the visits of the French researchers and students to the United States. US students will benefit from the exchange of scientific techniques and experience with an international research team.