Geomicrobiology of a high saline mine tailings in the desert Atacama, Chile

对智利阿塔卡马沙漠Bahía de Chañaral湾的高含盐硫化物尾矿存款的地质微生物学和微生物生态学进行全面调查。利用催化报告沉积-荧光原位杂交（CARD-FISH）和实时定量PCR（Q-PCR）对5个采样点进行了深度依赖的微生物群落定量分析，旨在了解干旱气候下高盐度条件下Fe（II）-和硫氧化以及Fe（III）-和硫酸盐还原过程中的生物地球化学相互作用。采用变性梯度凝胶电泳（DGGE）和随后的16 S rRNA基因测序对微生物多样性进行深度依赖性分析，旨在鉴定高盐尾矿中的相关微生物。为了使生物采矿与水的高盐度，富集和耐盐Fe（II）氧化微生物的尾矿分离将进行分析和应用在生物浸出实验中，黄铁矿和黄铜矿在不同的盐浓度。

The geomicrobiology and microbial ecology of a high saline, sulfidic mine tailings deposit in the bay Bahía de Chañaral, Atacama desert, Chile shall be comprehensively investigated. A depth dependent quantitative microbial community analysis for five sampling sites using catalyzed reporter deposition - fluorescence in situ hybridisation (CARD – FISH) and quantitative real-time PCR (Q-PCR) aims to understand the biogeochemical interactions during Fe(II)- and sulfuroxidation and Fe(III)- and sulfate-reduction at high salinity in arid climate. The depth dependent analysis of the microbial diversity using denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing of 16S rRNA genes aims to identify the relevant microorganisms in the high saline mine tailings. To enable biomining with water of high salinity, enrichments and isolates of halotolerant Fe(II)-oxidizing microorganisms from the tailings will be analysed and applied in bioleaching experiments with pyrite and chalcopyrite at varying salt concentrations.