NER: Hierarchical self-assembly of DNA tiles

我们将做什么：五个层次的自组装将用于控制单个纳米粒子和无机分子在数百微米大小的区域上的位置。自组装和酶处理步骤将被用于组装DNA瓦片1和具有4-60 nm尺寸范围的尺寸的多瓦片“筏”。瓦片将在已知的空间位置包含衍生化位点，以允许非DNA组分的附着。多达六种不同的分子或纳米颗粒可以附着到每个DNA瓦片上。分子剥离2将用于指导DNA筏与光刻特征（例如30 nm线）的结合。DNA筏的尺寸与光刻特征的尺寸相似，因此附着到筏上的单个分子将以很大的控制放置在表面上，并且可以位于其他光刻结构附近。这些能力将是非常有用的分子量子点元胞自动机电路和其他分子电子器件的建设。智力优点：该项目将：-探索分层设计作为一种工具，创造超分子的复杂性-扩展分子剥离，以前只用于小无机分子，生物分子，如DNA。- 整合自上而下和自下而上的方法来制造纳米至微米尺寸的结构该提案将对局部物理结构的详细控制与超高分辨率纳米光刻相结合，以创建大规模实施分子电子学不同架构所需的非重复阵列。3 DNA将用作有源元件的自组装电路板，其可以包括纳米颗粒、其它生物分子和小的有机或无机分子。这种方法可用于构建技术上有用的设备，例如与硅芯片上的I/O结构集成的分子电子现场可编程门阵列。

What we will do: Five levels of hierarchical self-assembly will be used to control the placement of single nanoparticles and inorganic molecules over areas that are hundreds of microns in size. Self-assembly and enzymatic processing steps will be used to createDNA tiles1 and multi-tile "rafts" that have dimensions in the 4-60 nm size range. The tiles will contain derivatization sites at known spatial locations to permit attachment of non-DNA components. Up to six different molecules or nanoparticles could be attached to each DNA tile. Molecular liftoff2 will be used to direct the binding of the DNA rafts to lithographic features, such as 30 nm lines. The dimensions of the DNA rafts are similar to the dimensions of the lithographic features, so individual molecules that are attached to the rafts will be placed on the surface with great control and could be located near other lithographic structures. These capabilities would be very useful for construction of molecular quantum-dot cellular automata circuits and other molecular electronic devices.Intellectual Merit: This project will: -explore hierarchical design as a tool for creation of supramolecular complexity -extend molecular liftoff, which has previously been used only for small inorganic molecules, to biomolecules such as DNA. -integrate top-down and bottom-up approaches to the fabrication of structures on the nanometer to micron size scaleBroader impact: This proposal combines detailed control over local physical structure with ultra-high resolution nanolithography to create non-repetitive arrays of the types required for large-scale implementation of different architectures for molecular electronics.3 DNA will be used as a self-assembling circuit board for active components, which could include nanoparticles, other biomolecules, and small organic or inorganic molecules. This method could be used to construct technologically useful devices, such as molecular electronic field-programmable gate arrays that are integrated with I/O structures on a silicon chip.