New Transmission Electron Microscope for a Core EM Facility

该合同支持购买配备低温转印支架、旋转支架和ccd摄像机的电子显微镜。该系统将取代生物系电子显微镜设施中已有20年历史的仪器。该设施目前拥有扫描和透射式电子显微镜以及先进的光学显微镜。一个由EM设施主任、副主任和部门主席组成的咨询委员会监督文书的操作和维护。新的透射电子显微镜的增强能力将有助于11个不同实验室进行不同的结构研究，包括对生活在高盐度水域的单细胞光合作用有机体的研究；线粒体蛋白质的定位和表征；收缩蛋白质功能的分析；鱼类进化适应的研究；与珊瑚有关的微生物的鉴定；以及DNA重组分析。该仪器的使用将为各种基本的细胞生物学和进化问题提供新的见解，并在少数族裔服务机构的本科生培训中发挥重要作用。除了这些研究和培训角色，显微镜设施还参加一年一度的科学开放日，向来自大圣地亚哥地区的数百名社区成员介绍该机构的学生和教职员工所做的研究。

This award provides support for purchase of a Transmission Electron Microscope equipped with cryotransfer holder, rotation holder, and CCD camera. The system will replace a 20 year old instrument located in the Electron Microscope Facility in the Department of Biology. The Facility presently houses both scanning and transmission electron microscopes as well as advanced light microscopes. An advisory committee, composed of the EM Facility Director, the Associate Director, and the Department Chair, supervises operation and maintenance of instruments. The enhanced capabilities of the new TEM will benefit diverse structural studies by 11 different laboratories, including studies of a unicellular photosynthetic organism that lives in hypersaline waters; localization and characterization of proteins in mitochondria; analysis of contractile protein function; study of evolutionary adaptations in fish; identification of microbes associated with corals; and analysis of DNA recombination. Use of the instrument should provide new insights into diverse basic cell biological and evolutionary questions, as well as playing an important role in the training of undergraduate students at a minority serving institution. In addition to these research and training roles, the microscopy facility also participates in an annual Sciences Open House that introduces hundreds of community members from the greater San Diego area to the research done by students and faculty at the institution.