2010 Project: Identification and Functional Analysis of Genes Required for Gametophyte Development

0313501 Sundaresan拟议的研究将确定和确定拟南芥配子体基因的功能。 将使用Ds插入系进行配子体突变的突变体筛选，从而鉴定估计400个配子体突变体。 将通过序列鉴定被这些插入破坏的基因，并将其发布在UC Davis的网站上（http：//www-plb.ucdavis.edu/Labs/sundar/）。 将通过对突变体中的雌配子体和雄配子体进行综合遗传、细胞和发育研究来表征基因功能。 表征的突变体将通过在ABRC储备中心的沉积而公开提供。此外，我们将使用表达谱来识别雌配子体特异性基因。 对于开花植物配子体发育所需的基因和功能知之甚少。2010年项目的目标是确定所有拟南芥基因的功能，这将需要确定和鉴定配子体基因，这在植物繁殖中发挥关键作用。这些研究也将产生潜在的应用控制作物的繁殖。更广泛的影响：该项目将为两个参与机构的博士后研究员、研究生和本科生提供植物生物学研究方面的全面培训。 将努力从代表性不足的群体中征聘个人参加这一项目。 在本科生的情况下，PI将在其机构的具体方案，旨在鼓励少数民族参与研究的目的。 此外，该项目的参与者将通过高中学生的实习和通过在中学的讲习班单元参与学校的外联工作。

0313501SundaresanThe proposed research will identify and determine functions of gametophytic genes in Arabidopsis. A mutant screen for gametophytic mutations using Ds insertion lines will be performed, resulting in the identification of an estimated 400 gametophytic mutants. The genes disrupted by these insertions will be identified by sequence and posted on a website at UC Davis (http://www-plb.ucdavis.edu/Labs/sundar/). Characterization of gene function will be carried out through integrated genetic, cellular and developmental study of the female and male gametophytes in the mutants. The characterized mutants will be made publicly available through deposition in the ABRC stock center. In addition, we will use expression profiling to identify female gametophyte-specific genes. Relatively little is known about the genes and functions required for gametophytic development in flowering plants. The aim of the 2010 project to determine the functions of all Arabidopsis genes will require the identification and characterization of gametophytic genes, which play a critical role in plant reproduction. These studies will also yield potential applications towards the control of reproduction in crop plants. Broader Impact: The project will provide comprehensive training in plant biology research for postdoctoral fellows, graduate students and undergraduate students at both participating institutions. Efforts will be made to recruit individuals from underrepresented groups to participate in this project. In the case of undergraduate students, the PIs will work with specific programs at their institutions intended for the purpose of encouraging minority participation in research. In addition, participants in the project will be involved in outreach efforts to schools through the attachment of high school students and through workshop modules in middle schools.