Biophotonics: Molecular Motor Biophotonics

这项建议的具体目的是：1)设计和构建用于运输和分选微管的运动蛋白分子马达连接的微加工通道。2)将荧光标记的DNA寡核苷酸(分子信标)作为诱饵附着到微管上，通过荧光显微镜可视化核酸的结合和运输。3)制作单片封装的有机发光二极管(OLED)，耦合到微管微通道作为局部源来激发荧光标记。4)制造用于局部荧光信号检测的集成光检测器。光电探测器将与有机发光二极管结合使用，以检测结合到在通道中移动的微管的荧光DNA。5)组装一个系统，该系统由连接到传输通道的样本库、检测器、用于从空的微管中分拣货物的分叉和用于存储所选感兴趣的分子的收集库组成。

0323024HancockThe specific aims of this proposal are to:1) Design and construct microfabricated channels to which kinesin molecular motors are attached for transporting and sorting microtubules.2) Attach fluorescently labeled DNA oligonucleotides (molecular beacons) to microtubules as bait, visualize binding and transport of the nucleic acid cargo by fluorescence microscopy.3) Fabricate monolithically encapsulated organic light emitting diodes (OLEDs) with coupling to the microtubule microchannels as local sources to excite the fluorescent labels.4) Fabricate integrated photodetectors for local detection of fluorescence signals. The photodetectors will be used in combination with the OLEDs to detect fluorescent DNA bound to microtubules traveling in the channels.5) Assemble a system consisting of a sample reservoir connected to a transport channel, a detector, and bifurcation to sort cargo-laden from empty microtubules, and a collection reservoir to deposit the selected molecules of interest.