SBIR Phase I: Portable SI-HPLC Analyzer

这个小企业创新研究（SBIR）第一阶段项目提出了一种便携式全自动色谱分析仪的开发。该分析仪将集成几种经过验证的技术，以生产一种紧凑，坚固且易于在线，现场或现场使用的自动化仪器，特别是在需要复杂的HPLC（高效液相色谱）分析的地方。基于顺序进样（SI）协议和高效液相色谱仪器，该仪器将完全自动化，并提供关于样品收集，预处理，化学修饰，分离和检测目标分析物的综合方法。该化学分析仪将利用一系列新技术，包括顺序注射，便携式高压注射泵和溶胶-凝胶高效液相色谱柱，在其开发的这种混合分析仪被称为高效液相色谱（SI-HPLC）的顺序注射。尽管HPLC是许多实验室的基本设备；由于其整体尺寸大，功耗高，并且在分析前需要手动样品再处理/制备，因此它在很大程度上仍然是一个台式仪器。通过集成几种不断发展的技术，高效液相色谱分析可以完全自动化，并在更低的压力下进行，这也将使该分析仪非常紧凑，真正便携以及节能。拟议的SI-HPLC仪器将用于在线过程控制，现场环境监测或作为医疗，执法或军事用途的多用途现场准备分析仪。SI-HPLC仪器将是一种低成本、便携的系统，因为它将依赖于一个开放的架构，通过软件可编程性使其成为可能，因此可以高度适应不同的应用。顺序进样技术使该分析仪非常适合未经培训的人员使用或远程自主分析，因为样品处理和制备可以完全自动化。SI-HPLC将用于在线过程控制（例如制药），为质量控制或最佳产品收率提供实时反馈；对水源的农药或渗滤液污染进行现场监测；或者作为移动医院单元的现场准备分析仪。SI-HPLC仪器将有广泛的应用，因为它将广泛接受/期望的HPLC方法纳入一个完全便携式和具有成本效益的现场分析仪。

This Small Business Innovation Research (SBIR) Phase I project proposes the development of a portable and fully automated chromatographic based analyzer. This analyzer will integrate several proven technologies to produce an automated instrument that is compact, robust and easily implemented for on-line, at-site, or field-ready use, especially where complex HPLC (High Performance Liquid Chromatography) analyses are needed. Based on Sequential Injection (SI) protocol and HPLC instrumentation, this instrument will be full automated and provide an integrated approach with respect to sample collection, pretreatment, chemical modification, separation and detection of target analytes. This chemical analyzer will exploit several novel technologies including sequential injection, portable high-pressure syringe pumps and sol-gel HPLC columns in its development of this hybrid analyzer referred to as Sequential Injection for High performance Liquid Chromatography (SI-HPLC). Although HPLC is a fundamental fixture in many laboratories; it remains largely a bench-top instrument due to its overall large size, high power consumption and need for manual sample retreatment/preparation prior to analysis. By integrating several evolving technologies, HPLC analysis can be fully automated and conducted at much lower pressures, which will also make this analyzer very compact and truly portable as well as energy efficient. The proposed SI-HPLC instrument will find applications at on-line process control, at-site environmental monitoring or as a multipurpose field-ready analyzer for medical, law-enforcement or military use. The SI-HPLC instrument will be a low cost, portable system that is highly adaptable to different applications since it will rely on an open architecture made possible by software programmability. Sequential Injection technologies make this analyzer ideally suited for use by untrained personnel or for remote autonomous analysis since sample handling and preparation can be completely automated. Examples for SI-HPLC use will be for online process control (e.g. pharmaceuticals) to provide real-time feedback for Quality Control or optimal product yield; at-site monitoring of pesticide or leachate contamination of water sources; or as a field-ready analyzer for mobile hospital units. The SI-HPLC instrument will have broad application since it will employee widely accepted/desired HPLC methodologies into a completely portable and cost-effective field analyzer.