SBIR Phase II: Portable Sequential Injection (SI)-High Performance Liquid Chromatography (HPLC) Analyzer

小型企业创新研究（SBIR）第二阶段研究项目旨在开发便携式全自动色谱分析仪。该分析仪将集成几种成熟的技术，以生产一种紧凑、坚固且易于在线、现场或现场使用的自动化仪器，特别是需要复杂HPLC（高效液相色谱）分析的地方。基于顺序注射（SI）方案和HPLC仪器，该设备将完全自动化，并提供关于样品收集，预处理，化学修饰，分离和检测目标分析物的集成方法。该化学分析仪将采用几种新技术，包括顺序注射，便携式高压注射泵和溶胶-凝胶HPLC柱在其发展。拟议的SI-HPLC仪器将在在线过程控制，现场环境监测或作为一个多用途的现场准备分析仪用于医疗，执法和军事用途的应用。顺序进样技术使该分析仪非常适合未经培训的人员使用或远程自动分析，因为样品处理和制备可以完全自动化。SI-HPLC的初始目标用途将用于在线生物过程控制（例如制药），为质量控制或最佳产品产量提供实时反馈。

This Small Business Innovation Research (SBIR) Phase II research project is aimed at the development of a portable and fully automated chromatographic based analyzer. This analyzer will integrate several proven technologies to produce an automated instrument that is compact, robust and easily implemented for on-line, at-site, or field-ready use, especially where complex HPLC (High Performance Liquid Chromatography) analyses is needed. Based on both Sequential Injection (SI) protocol and HPLC instrumentation, this device will be fully automated and provide an integrated approach with respect to sample collection, pre-treatment, chemical modification, separation and detection of target analytes. This chemical analyzer will exploit several novel technologies including sequential injection, portable high-pressure syringe pumps and sol-gel HPLC columns in its development. The proposed SI-HPLC instrument will find applications in on-line process control, at-site environmental monitoring or as a multipurpose field-ready analyzer for medical, law-enforcement and military use. Sequential Injection technologies make this analyzer ideally suited for use by untrained personnel or for remote autonomous analysis since sample handling and preparation can be completely automated. Initial targeted use for the SI-HPLC will be for online bioprocess control (e.g. pharmaceuticals) to provide real-time feedback for Quality Control or optimal product yield.