GOALI: Development of Combinatorial Polymeric Substrates for Efficient Screening of Protein Adsorption

提案标题：GALI：开发用于有效筛选蛋白质吸附的组合聚合物底物提案编号：CTS-0403535主要研究员：Jan Genzer研究所：北卡罗来纳州立大学这项提案的目标是开发一种新的技术，用于研究蛋白质在材料表面的吸附。一种改进现有分析工具的组合筛选方法将使蛋白质吸附的评估更快、更可靠、更有效。这种方法将提供一种有效的分析方法，并将促进具有可控修饰的生物界面特性的器件的制造。基于接枝到平板二氧化硅衬底上的大分子的量身定制沉积的新型聚合物表面将被制备和表征。将控制条件，以允许在聚合物接枝密度和接枝聚合物链长中形成连续的梯度。将制备在其上接枝聚合物的接枝密度和链长在垂直方向上独立变化的梯度结构。就更广泛的影响而言，教育努力将侧重于在北卡罗来纳州芒特艾里的一所小学开展旨在吸引当地K-12教师和学生的活动。该计划中开发的基于梯度的技术将提供一种强大的方法，该方法应该优于当前的技术，因为它使蛋白质吸附的快速和简单筛选以及对广泛参数空间的完整和可靠的探索成为可能。该计划中开发的结构将促进细胞的受控吸附，这一过程代表了许多制药设备的中心焦点。

Proposal Title: GOALI: Development of Combinatorial Polymeric Substrates for Efficient Screening of Protein AdsorptionProposal Number: CTS-0403535Principal Investigator: Jan GenzerInstitution: North Carolina State UniversityThe objective of this proposal is to develop a novel technology for investigating protein adsorption on material surfaces. A combinatorial screening methodology that improves upon existing analytical tools will enable faster, more reliable, and more efficient evaluation of protein adsorption. This approach will provide an effective analytical method and will facilitate manufacturing of devices with controllably modified biointerfacial properties. Novel polymeric surfaces based on the tailored deposition of macromolecules grafted to flat silica substrates will be fabricated and characterized. Conditions will be controlled to permit formation of continuous gradients in polymer grafting densities, and graft-polymer chain length. Gradient structures, on which the polymer grafting density and chain length of the grafted polymers vary independently in orthogonal directions, will be prepared. In terms of the broader impacts, educational efforts will focus on activities in an elementary school in Mount Airy, NC aimed at attracting local K-12 teachers and students. The gradient-based technology developed in this program will provide a powerful methodology that should be superior to current techniques in that it enables fast and simple screening of protein adsorption and complete and reliable exploration of a broad parameter space. The structures developed in this program will facilitate controlled adsorption of cells, a process that represents the central focus of many pharmaceutical devices.