Acquisition of a Fluorescent Activated Cell Sorter (FACS) for SDSU Biology Department

在Kathleen McGuire博士的指导下，圣地亚哥州立大学获得了一笔赠款，通过购买荧光激活细胞分选仪（FACS），部分支持在SDSU建立流式细胞仪设备。生物学中五个主要用户的研究目前需要使用流式细胞术，流式细胞术是一种允许对单个细胞进行各种特征和特征分析的技术。一种足够复杂的仪器将被购买并放置在生物系，以服务于SDSU的教师多年。这台流式细胞仪将是SDSU同类仪器中的第一台，也是该资助的五个共同项目负责人提出的许多实验中唯一可用的仪器。生物系的其他几位教员也将使用该仪器，用于多种研究领域，如不同沙门氏菌菌株的宿主特异性、海鞘动物的无索发育、骨骼和心脏肌肉的结构和功能。该设施还将为化学系的教师和新成立的微生物研究中心的成员提供服务。该设施的两个独特方面是生态学家以及细胞和分子生物学家和生物化学家的使用，以及原核和真核细胞的使用。流式细胞仪设施还将允许本科生和研究生进行广泛的实践培训，并允许将FACS的使用纳入生态学、微生物学、细胞和分子生物学的研究生和本科生课程。由于约44%的生物学专业学生和23%的SDSU研究生属于少数族裔，因此他们可以很容易地获得这些设施和仪器。通过收购SDSU的流式细胞仪设备，上述每个研究项目都将得到显著增强，校园内的许多其他研究项目也是如此。圣地亚哥州立大学的学生将首次有机会学习这项重要的技术，并能够获得技能，为他们提供更好的工作机会，并使他们能够以新的方式为大圣地亚哥地区和加利福尼亚州的大型生物技术产业做出重大贡献。

A grant has been awarded to San Diego State University under the direction of Dr. Kathleen McGuire for partial support for the establishment of a flow cytometry facility at SDSU through the purchase of a fluorescence-activated cell sorter or FACS. The research of five major users in Biology currently requires the use of flow cytometry, a technology that allows single cells to be analyzed for a variety of traits and characteristics. An instrument that is sophisticated enough to serve the faculty at SDSU for many years to come will be purchased and placed in the Biology department. This flow cytometer will be the first instrument of its kind at SDSU and the only one available for many of the experiments proposed by the five co-PIs of the grant. Several other faculty in Biology will also use the instrument, for research areas as diverse as the host specificity of different strains of Salmonella, the development of notachords in Ascidians, and skeletal and heart muscle structure and function. The facility will also service faculty from the department of Chemistry and members of the newly established Center for Microbial Studies. Two unique aspects of this facility will be its use by ecologists as well as cell and molecular biologists and biochemists, and its use for both prokaryotic and eukaryotic cells. The flow cytometry facility will also permit extensive undergraduate and graduate student hands-on training and allow the use of the FACS to be incorporated into both the graduate and undergraduate curriculum in ecology, microbiology and cell and molecular biology. Because ~44% of Biology majors and 23% of SDSU's graduate students belong to underrepresented minority groups, their access to this facility and instrumentation will be readily achieved. Each of the research programs outlined above will be significantly enhanced by the acquisition of a flow cytometry facility at SDSU, as will many other research programs across campus. The students at SDSU will be offered, for the first time, opportunities to learn about this important technology and will be able to gain skills that will provide them with better job opportunities and allow them to significantly contribute in a new way to the large biotechnology industry in the greater San Diego area and the State of California.