SBIR Phase II: Rapid Detection of Bacterial Contaminants Using Micro-Fluidic Biochips

这个小企业创新研究（SBIR）二期项目将开发一种基于微流体的系统，以细菌的介电浓度作为中间步骤，用于检测活病原体。该系统将使用第一级浓缩器，然后是介电泳浓缩，最后在具有集成阻抗测量的培养基中培养以检测培养生长。该项目的商业应用将是在生物制药生产操作中检测水生微生物。所提出的方法将在不到3小时内电子检测水样中微生物的活力，而不像目前的技术需要2-7天才能产生结果。

This Small Business Innovation Research (SBIR) Phase II project will develop a microfluidic based system for the detection of viable pathogens using dielectric concentration of bacteria as an intermediate step. This system would use a first-stage concentrator, followed by dielectrophoretic concentration, and finally by culturing in media with integrated impedance measurements to detect culture growth. The commercial application of this project will be on the detection of waterborne microorganisms in biopharmaceutical manufacturing operations. The proposed method would electronically detect the viability of microorganisms in water samples in less than 3 hours, unlike the current technology that takes 2-7 days to yield results.