Tissue- and organ specificity of jasmonate responses in the development of tomato flowers

茉莉酸（JA）及其衍生物，统称为茉莉酸酯，是脂质衍生的信号分子，参与防御病原体和响应草食动物的伤害，调节植物与微生物的互利相互作用，而且还参与植物生长和发育。它们中的许多在调控网络中积极或消极地改变基因表达。关于JA在番茄花发育中的作用的资料很少。因此，我们计划在花发育过程中记录：（i）各种花器官中生物活性茉莉酸的量和发生，以及（ii）茉莉酸相关基因在雌性和雄性生殖组织中的表达和代谢产物积累。将使用以下补充方法：（a）详细的茉莉酸剖析解剖花器官，（B）茉莉酸的免疫细胞化学检测，使用高度特异性JA抗体，以可视化生物活性茉莉酸组织特异性，和（c）转录和代谢产物剖析的生殖组织分离的野生型和jai 1，突变体在JA感知缺陷，从而已知表现出缺陷的女性发展。为此，我们将确定茉莉酸依赖的调节成分和代谢途径，对番茄生殖器官的正常发育至关重要。

Jasmonic acid (JA) and its derivatives, collectively known as jasmonates, are lipid-derived signaling molecules involved in defense against pathogens and in response to wounding by herbivores, in the regulation of mutualistic interactions of plants with microorganisms, but also in plant growth and development. Many of them alter gene expression positively or negatively in a regulatory network. Only few data exist on the function of JA in development of tomato flowers. Therefore, we plan to record during flower development: (i) the amount and the occurrence of bioactive jasmonates in various flower organs, and (ii) the jasmonaterelated gene expression and metabolite accumulation in female and male reproductive tissues. The following complementary approaches will be used: (a) detailed jasmonate profiling of dissected flower organs, (b) immuno cytochemical detection of jasmonates using a highly specific JA antibody to visualize bioactive jasmonates tissue specifically, and (c) transcript and metabolite profiling of reproductive tissues isolated from wild type and jai1, a mutant defective in JA perception, thereby known to exhibit defects in female development. To this end, we will identify JA-dependent regulatory components and metabolic pathways essential for proper development of reproductive organs in tomato.