Laboratory evolution of mRNA stability

本研究将探讨多基因操纵子中mRNA稳定性、翻译和蛋白质合成的调控机制。这将为平衡操纵子中多个基因的表达提供一种新的方法。PI已经开发了表达系统，允许在异源基因的转录和翻译位点之间引入二级结构，以保护mRNA免受核内溶解裂解导致mRNA和蛋白质水平增加的影响。mRNA降解有许多未知因素，更好地了解多基因操纵子对mRNA稳定性的控制是一个有价值的目标，可能导致发现新的mRNA降解机制。这项工作将使用实验室技术来进化操纵子中多个基因的期望表达水平和/或mRNA的新稳定性决定因素。该技术可用于生产多亚基蛋白，例如用于疫苗。

0439124KeaslingThe proposed research will examine the mechanisms regulating mRNA stability, translation, and protein synthesis in multi-gene operons. It will develop a new method for balancing the expression of multiple genes in operons. The PI has developed expression systems that allow for the introduction of secondary structures between the transcription and translation sites of heterologous genes to protect the mRNA from endonucleolytic cleavage resulting in increases in both mRNA and protein levels. There are many unknowns in mRNA degradation and a better understanding of the control of mRNA stability in multi-gene operons is a worthy objective that may result in the discovery of new mRNA degradation mechanisms.. This work will use laboratory techniques to evolve the desired levels of expression of multiple genes in an operon and/or new stability determinants for mRNA.This technology may find use for the production of multi subunit proteins such as used for vaccines.