Analysis of differential functional capacity and origins of memory B-lymphocyte subsets

免疫记忆是适应性免疫反应的一个重要特征。最近，M.Shlomchik博士的实验室表明，记忆B淋巴细胞由明确的表型亚群组成，可以通过细胞表面标记CD80、PD-L2和CD73的特定表达来识别。通过使用NP特异性小鼠记忆B细胞系统，它在免疫后提供大量抗原特异性记忆B细胞，我将在拟议的团契中进一步描述这些新描述的亚群。主要目的是确定它们的来源，并测试记忆B细胞亚群是否具有不同的功能能力。将进行实验，以确定刺激的类型和程度是否影响所产生的记忆B细胞的子集类型。将对亚群进行纯化，以鉴定体外和体内的增殖和分化能力。这些亚群将使用基因表达谱、RT-qPCR和流式细胞仪进行进一步的解剖，将这些亚群相互比较并与幼稚细胞进行比较，以获得对差异功能的洞察。这种记忆B细胞亚群的描绘应该为了解次级B细胞反应的功能提供重要的洞察力。

Immunological memory is a defining feature of the adaptive immune response. Very recently it was shown in the laboratory of Dr. M. Shlomchik that memory B-lymphocytes are comprised of clear phenotypic subsets that can be identified by specific expression of the cell-surface markers CD80, PD-L2 and CD73. By using NP-specific murine memory B cell systems, which provide a large number of antigen-specific memory B cells after immunization, I will further characterize these newly described subsets in the proposed fellowship. Main objectives are to determine their origins and to test whether memory B cell subsets have differential functional capacity. Experiments will be performed to address whether type and degree of stimulation influences the subset type of memory B cells generated. Subsets will be purified to identify proliferative and differentiative capacity in vitro and in vivo. These subsets will be further dissected using gene expression profiling, RT-qPCR, and FACS, comparing the subsets to each other and to naïve cells in order to obtain insight into differential function. The delineation of such memory B cell subsets should provide important insight into how secondary B cell responses function.