CAREER: Linking Tissue Nano-Structure and Molecular Biology to Physiological Function using Nonlinear Optical Microscopy

0448950叶摘要这个职业发展计划的目的是整合在纳米，微米和宏观尺度的多维研究，为一个系统的方法，以复杂的生物医学问题，连接分子细胞生物学和化学的生理（dys）功能。通过将非线性光学显微镜（NLOM）与电子显微镜、基因组分析、生物力学应力应变和临床体内测量相结合，将实现组织光学、机械生物学和光学清除的多维研究的整合。 NLOM是一种非破坏性的激光扫描显微镜技术，它可以在生物事件过程中对厚的活组织标本中的（亚）细胞过程进行成像，而无需固定，染色或切片。 通过NLOM的组织成分的特定成像将经由非线性光学光谱学（例如，二次谐波产生和双光子激发荧光）。 NLOM将用于监测组织结构、组织、组成（亚）细胞分辨率以及由于机械和化学环境改变以及生长和重塑而引起的变化。

0448950YehABSTRACTThis CAREER-development plan aims to integrate multidimensional studies at nano-, micro-, and macroscales for a systems based approach to complex biomedical problems linking molecular cell biology and chemistry to physiological (dys)function. Integration of multidimensional studies in tissue optics, mechanobiology and optical clearing will be achieved by interfacing nonlinear optical microscopy (NLOM) with electron microscopy, genomic profiling, biomechanical stress-strain and clinical, in vivo measurements. NLOM is a non-destructive, laser scanning microscopy technique, which can image (sub-) cellular processes in a thick, living tissue specimen over the course of a biological event without fixing, staining or sectioning. Specific imaging of tissue constituents by NLOM will be accomplished via nonlinear optical spectroscopy (e.g., second-harmonic generation and two-photon excited fluorescence). NLOM will be used to monitor tissue structure, organization, composition with (sub-) cellular resolution and changes due to altered mechanical and chemical environments and growth and remodeling.