A Genome-Wide Optical Map of Maize

55利用光学作图系统构建玉米(玉米)基因组(自交系B73)的物理图谱，图谱覆盖率为300倍。光学作图是一种集成的、高通量的单分子系统，通过对大量全基因组的分析已经证明了它的优点。直接从细胞中提取的超长DNA分子将被分析。一个典型的分子大约500kb，或大约200微米长，自动光学显微镜将被用来对这些分子进行成像。因此，将使用光学测绘系统获取约1500,000个单分子图谱，并将构建覆盖整个基因组的图谱。DNA序列数据将与这张地图进行比较，以帮助拼凑这些信息，以涵盖这个非常庞大和复杂的基因组。玉米基因组的光学图谱将提供一种资源，大大促进玉米基因组信息的发现，玉米是一种至关重要的动物饲料和能源作物。光学测绘非常适合作为跨多个学科的教学、培训和学习的主题，并将加强植物基因组学的科学培训。在这个玉米基因组项目中，少数群体中的几个成员将发挥积极作用，研究玉米基因组光学图谱的研究人员将帮助举办外联讲习班、课程和植物基因组分析课程。通过威斯康星州苏必利尔市的联系人，美国原住民初中生和高中生将来到威斯康星州麦迪逊大学参加植物基因组学暑期课程，课程内容来自玉米光学图谱。由于这笔赠款，威斯康星大学麦迪逊分校和其他几个机构的计算机科学、工程学、作物基因组学、数学等领域的合作者网络将得到加强。玉米基因组光学图谱和相关数据将通过一个项目网站(可通过http://www.lmcg.wisc.edu/index.html)，玉米地理数据库http://www.maizegdb.org/)和谷物(http://www.gramene.org/).)获取

55A physical map of the maize (corn) genome (inbred line B73) will be constructed by the Optical Mapping System with 300 fold map coverage. Optical Mapping is an integrated, high-throughput single molecule system that has proven its merit by the analysis of a large number of whole genomes. Very long DNA molecules, directly extracted from cells will be analyzed. A typical molecule is approximately 500 kb, or about 200 microns in length, and automated light microscopy will be used to image these molecules. Consequently ~1,500,000 single molecule maps will be acquired using an Optical Mapping System and a map will be constructed, to cover the entire genome. DNA sequence data will be compared with this map to aid in the piecing together of such information to cover this very large and complex genome. The Optical Mapping of the maize genome will provide a resource that will significantly advance discovery of genomic information in maize--a critically important crop for animal feed and energy. Optical Mapping is ideally suited as a topic for teaching, training and learning across multiple disciplines, and will strengthen scientific training in plant genomics. Several members of underrepresented minority groups will be active in this maize genome project, and researchers working on the Maize Genome Optical Map will help conduct Outreach workshops, classes, and a course on Plant Genome Analysis. Through contacts in Superior, WI, Native American middle and high school students will come to UW-Madison for a summer course on plant genomics, drawing from Optical Mapping of Maize. A network of collaborators in computer science, engineering, crop genomics, mathematics at the Unerivery of Wisconsin, Madison and at several other institutions will be strengthened as a result of this grant. The Maize Genome Optical Map and associated data will be made accessible through a project web site (accessible via http://www.lmcg.wisc.edu/index.html),Maize GDB http://www.maizegdb.org/) and Gramene (http://www.gramene.org/).