NER: Electroosmotic Flow and Single Molecule Trapping in Hybrid Nanochannels

摘要提案ID：0507903标题：NER：杂化纳米通道中的电渗流和单分子捕获PI：Deyu LiInst：范德比尔特大学Nanofluidics，即纳米系统中流体行为和运动的研究，仍处于起步阶段。 生物分子传感、测序和分离迫切需要在这一领域取得重大进展。 我们将利用研究员在纳米技术和流体力学方面的经验，制作具有不同壁材的混合纳米通道，研究这些纳米通道中的电渗流，并探索利用这些纳米通道主动捕获单个生物分子的可行性。 PI将开发制造过程，以制造两种类型的纳米通道：SiO2通道和以SiO2和Al2O3作为壁材料的混合通道。 这些纳米通道内的电渗流将通过测量电流和检测荧光信号来研究。 将进行理论预测来解释实验观察结果。 PI将使用这些纳米通道来探索纳米通道内世界上最小的再循环流。 拟议的研究将推进对具有纳米限制的电渗流的理解，并为纳米通道内的单生物分子操作提供可行性研究，这将扩展药物发现研究的前沿。

ABSTRACTProposal ID: 0507903Ttile: NER: Electroosmotic flow and Single Molecule Trapping in Hybrid NanochannelsPI: Deyu LiInst: Vanderbilt UniversityNanofluidics, the study of fluid behavior and motion in nanoscale systems, is still in its infancy. Significant advances in this field are urgently needed for biomolecular sensing, sequencing, and separation. We will fabricate hybrid nanochannels with different wall materials, investigate electroosmotic flow in these nanochannels, and explore the feasibility of active trapping of single biomolecules with these nanochannels.The proposed work is based on the PIs' previous experience in nanotechnology and fluid mechanics. The PIs will develop the fabrication process to make two types of nanochannels: SiO2 channels and hybrid channels with both SiO2 and Al2O3 as wall materials. Electroosmotic flow inside these nanochannels will be investigated by measuring the electric current and detecting fluorescent signals. Theoretical predictions will be carried out to explain the experimental observations. The PIs will use these nanochannels to explore the world's smallest recirculation flow inside nanochannels. The proposed research will advance the understanding of electroosmotic flow with nano-confinement and provide a feasibility study for single biomolecule manipulation inside nanochannels, which will extend the frontier of drug discovery research.