Wafer-scale bio/nano filament assembly for chem/bio sensors

150字计划总结纳米技术中的一个主要挑战是纳米/生物分子在高堆积密度下的晶片规模组装，理解和建模细胞的机理是当前纳米技术中的主要挑战之一。将用浸没有限元方法分析复合电场及其对分子的作用力，以实现分子的组装。成像方法将结合使用，以调查组装结果。，但也可能是最有成效的任务，是本世纪和未来几十年的任务。这项任务很复杂，因为力学行为是通过一系列尺度的相互作用而出现的，对于模型来说，反映这些尺度是必不可少的，这样它们最终才能成为能够预测各种力学行为的“第一原则”模型。将开发的3D模拟工具将跨越三个无尺度：(1)晶片规模的纳米/生物分子的组装；(2)生物细丝悬浮液的力学；以及能够通过扫描电子显微镜、原子力显微镜和原子力显微镜进行成像的成像工具的开发；(3)用于组装过程建模的建模模拟工具的开发；以及(4)对电场驱动力的理解。整个单元的粗粒度模型。将采用分层建模方法：a)将生物纤维的材料特性从原子尺度传递到生物纤维悬浮尺度，b)通过对生物纤维悬浮模拟进行粗粒化，将活性细胞材料的有效性质传递到细胞迁移的连续尺度模拟。将在两个层面上制定并行的多尺度耦合方案：a)。尽管信息将主要通过传递属性信息在尺度之间传递，但将开发出将连续介质力学与分子力学联系起来的并行耦合方案，使原子运动在某些应用中能够直接耦合到生物纤维特性，以及b)一种新的混合模拟方案，该方案通过执行生物纤维悬浮模拟来求解细胞运动的连续方程并同时解析局部细胞骨架结构。单个分子装置将被开发并用于生物/化学传感器的测试。我们小组正在制造的一种新型的测量细胞力的NEMS装置将被用于测量牵引力和同时成像细胞的纤维结构。这将启动分子规模生物/化学传感器的大规模开发，最终可能以批量生产的方式进行生产。细胞运动的模拟..此外，在更大的范围内，我们将开发新的模型来从生物纤维悬浮液的力学模型中获得粗粒度的主动应力，从而考虑生物流体-结构效应、静电相互作用、热行为以及聚合和解聚。

150 WORD PROJECT SUMMARYA major challenge in nanotechnology is wafer-scale assembly of nano/bio molecules with the high packing density Understanding and modeling the mechanics of the cell is one of the major challengesin current nanotechnology.. A composite electric field and the forces induced on molecules to achieve their assembly, will be will be analyzed with the inmmersedImmersed FEM method. Imaging methods will be accompaniedcombinedused to investigate the assembly results. , and yet probably most rewarding, tasks of the present centurynext few decades. The task is complex, for the mechanical behavior emerges through an interaction of a hierarchy of scales, and it is essential for models to reflect these scales so that they can eventually become "first-principles" models that enable predictions of a large variety of mechanical behavior. The intellectual merits will beare the following3D simulation tools to be developed will span three scaless: (1) wafer-scale assembly of nano/bio molecules; (2) mechanics of bio-filament suspensions; and development of imaging tools to enable imaging by for SEM,, AFTEM, and AFM; (3) development of modeling simulation tools for assembly process modeling; and (4) understanding of electric field driven forces. coarse-grained models of the entire cell. An hierarchical modeling approach will be taken bewhere: a) material properties of bio-filaments will be passed from the atomic scale to the bio-filament suspension scale, and b) the effective properties of the active cell material will be passed on to the continuum scale simulations of cell migration by coarse graining the bio-filament suspension simulations. Concurrent multiscale coupling schemes will be developed at two levels: a) . Although information will be transferred between the scales primarily by passing property information, concurrent coupling schemes linking continuum mechanics to molecular mechanics will be developed that enable direct coupling of atomistic motions to biofiber properties in some applications, and b) a new hybrid simulation scheme that solves the continuum equations for cell motion and concurrently resolves the local cytoskeletal structure by performing bio-filament suspension simulations.Individual molecular devices will be developed and tested foras bio/chemical sensors.A novel NEMS device to measure cellular forces, being fabricated in our group, will be used for the measurements of traction forces and the simultaneous imaging of the fibrous structure of the cell. This will initiate the development of molecular scale bio/chemical sensors in a massthat ultimately could be mass-producedproducible way. the simulation of cell motility.. Also, at a larger scale, we will develop new models to obtain coarse-grained active stresses from mechanical models of bio-filament suspensions, thereby accounting for bio-fluid-structure effects, electrostatic interactions, thermal behavior and polymerization and depolymerization.